Detailed information of TA system TA148347

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2788910..2789439
• Replicon: chromosome
• Accession: NZ_CP047854
• Organism: Staphylococcus aureus strain UP_1525

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: E3S68_RS13885
• Protein ID: WP_000621175.1
• Coordinates: 2789077..2789439 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: E3S68_RS13880
• Protein ID: WP_000948331.1
• Coordinates: 2788910..2789080 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3S68_RS13850	2783947..2784507	+	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-
E3S68_RS13855	2784715..2785194	+	480	WP_001287087.1	hypothetical protein	-
E3S68_RS13860	2785187..2786770	+	1584	WP_001294626.1	PH domain-containing protein	-
E3S68_RS13865	2786757..2787248	+	492	WP_001205912.1	PH domain-containing protein	-
E3S68_RS13870	2787252..2787611	+	360	WP_000581197.1	holo-ACP synthase	-
E3S68_RS13875	2787677..2788825	+	1149	WP_001281154.1	alanine racemase	-
E3S68_RS13880	2788910..2789080	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
E3S68_RS13885	2789077..2789439	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
E3S68_RS13890	2789788..2790789	+	1002	WP_160199200.1	PP2C family protein-serine/threonine phosphatase	-
E3S68_RS13895	2790908..2791234	+	327	WP_001052491.1	anti-sigma factor antagonist	-
E3S68_RS13900	2791236..2791715	+	480	WP_001190829.1	anti-sigma B factor RsbW	-
E3S68_RS13905	2791690..2792460	+	771	WP_001041111.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T148347 WP_000621175.1 NZ_CP047854:2789077-2789439 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T148347 NZ_CP063499:c2950010-2949837 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT148347 WP_000948331.1 NZ_CP047854:2788910-2789080 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT148347 NZ_CP063499:c2950334-2950038 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1