Detailed information of TA system TA148329

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2691673..2692202
• Replicon: chromosome
• Accession: NZ_CP047852
• Organism: Staphylococcus aureus strain UP_274

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: E3S71_RS13295
• Protein ID: WP_000621175.1
• Coordinates: 2691840..2692202 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: E3S71_RS13290
• Protein ID: WP_000948331.1
• Coordinates: 2691673..2691843 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3S71_RS13260	2686709..2687269	+	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-
E3S71_RS13265	2687478..2687957	+	480	WP_001287088.1	hypothetical protein	-
E3S71_RS13270	2687950..2689533	+	1584	WP_001294626.1	PH domain-containing protein	-
E3S71_RS13275	2689520..2690011	+	492	WP_001205910.1	PH domain-containing protein	-
E3S71_RS13280	2690015..2690374	+	360	WP_000581200.1	holo-ACP synthase	-
E3S71_RS13285	2690440..2691588	+	1149	WP_001281145.1	alanine racemase	-
E3S71_RS13290	2691673..2691843	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
E3S71_RS13295	2691840..2692202	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
E3S71_RS13300	2692552..2693553	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
E3S71_RS13305	2693672..2693998	+	327	WP_001052491.1	anti-sigma factor antagonist	-
E3S71_RS13310	2694000..2694479	+	480	WP_001190830.1	anti-sigma B factor RsbW	-
E3S71_RS13315	2694454..2695224	+	771	WP_001041107.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T148329 WP_000621175.1 NZ_CP047852:2691840-2692202 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T148329 NZ_CP063493:78433-78849 [Escherichia coli]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAACCTGAAGCATTGCTTAAGCACCT
GGAGCAGTCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGTGCCACCGGTC
CGAAGGCCTCGCCACGCCACGTGCAACTGGTTGACGCGTTCTGTGAGAGGCTCGATGCCGTCCTGCCGTGGGACCGCGCC
GCGGTGGATGCGACCACGGAGATTAAGGTGGCACTGCGTCTCGCCGGCACGCCGATCGGTCCGAACGACACGGCGATAGC
CGGACACGCCATCGCTGCCTGTGCCATTCTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT148329 WP_000948331.1 NZ_CP047852:2691673-2691843 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT148329 NZ_CP063493:78206-78436 [Escherichia coli]
ATGAGAACCGTATCCATTTTTAAAAATGGCAACAACCGTGCTATCCGCCTTCCACGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGCCCCGTCCGGCCGACCTGGGGCTCATTCCTGGAGCTAG
AAAAGGCCGATCCGGACTTTATGACGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGAGTTAACCTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1