Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2139247..2139776 Replicon chromosome
Accession NZ_CP047851
Organism Staphylococcus aureus strain UP_338

Toxin (Protein)


Gene name mazF Uniprot ID Q6GF05
Locus tag E3S74_RS10440 Protein ID WP_000621176.1
Coordinates 2139247..2139609 (-) Length 121 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID T1YCG8
Locus tag E3S74_RS10445 Protein ID WP_000948331.1
Coordinates 2139606..2139776 (-) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E3S74_RS10420 2136226..2136996 - 771 WP_001041107.1 RNA polymerase sigma factor SigB -
E3S74_RS10425 2136971..2137450 - 480 WP_001190825.1 anti-sigma B factor RsbW -
E3S74_RS10430 2137452..2137778 - 327 WP_001052491.1 anti-sigma factor antagonist -
E3S74_RS10435 2137897..2138898 - 1002 WP_000390829.1 PP2C family protein-serine/threonine phosphatase -
E3S74_RS10440 2139247..2139609 - 363 WP_000621176.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
E3S74_RS10445 2139606..2139776 - 171 WP_000948331.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
E3S74_RS10450 2139861..2141009 - 1149 WP_001281140.1 alanine racemase -
E3S74_RS10455 2141075..2141434 - 360 WP_000581197.1 holo-ACP synthase -
E3S74_RS10460 2141438..2141929 - 492 WP_001286801.1 PH domain-containing protein -
E3S74_RS10465 2141922..2143499 - 1578 WP_001294646.1 PH domain-containing protein -
E3S74_RS10470 2143492..2143971 - 480 WP_001287082.1 hypothetical protein -
E3S74_RS10475 2144180..2144740 - 561 WP_001092414.1 K(+)-transporting ATPase subunit C -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-110)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 121 a.a.        Molecular weight: 13469.74 Da        Isoelectric Point: 10.1654

>T148306 WP_000621176.1 NZ_CP047851:c2139609-2139247 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVVHQKN

Download         Length: 363 bp

>T148306 NZ_CP063492:c1087360-1086983 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGCATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGCATTACAGCCTTACGCTGAATGACACACCGTTCGCGGATGAACGTGTGATTGAGCAGCATATTG
AAGCGGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGAATTC
AGCCCCTGCACCCGCTCTCAGTTAATCAACAGCATTGATATCCTCCGGGCCCGCCGGGCGACCGGCCTGATGACCCGCAG
CCACTACAGAATGGTGAACAACATCACCCTGGGTAAACACTGTGAGGCGAAACAATGA

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6251.78 Da        Isoelectric Point: 3.8305

>AT148306 WP_000948331.1 NZ_CP047851:c2139776-2139606 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Download         Length: 171 bp

>AT148306 NZ_CP063492:c1087807-1087451 [Escherichia coli]
ATCGTGTCAGACACACTCTCCGACGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAGGCCCTGTTTTGG
CGCCCGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGCATCAGAGGCCGGTTCAGCGATGCGG
ATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGTGAACTGAATCCC
CGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTGTGGTTACGTTTA
TCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A168PYX0

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0VRZ1

References