Detailed information of TA system TA148302

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /HTH_19(antitoxin)
• Location: 1592752..1593540
• Replicon: chromosome
• Accession: NZ_CP047851
• Organism: Staphylococcus aureus strain UP_338

Toxin (Protein)

• Gene name: -
• Uniprot ID: A0A2I7Y5B3
• Locus tag: E3S74_RS07600
• Protein ID: WP_000525004.1
• Coordinates: 1593079..1593540 (+)
• Length: 154 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A0E7YIA0
• Locus tag: E3S74_RS07595
• Protein ID: WP_000333630.1
• Coordinates: 1592752..1593066 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3S74_RS07545	1588292..1589458	-	1167	WP_031834538.1	DUF2800 domain-containing protein	-
E3S74_RS07550	1589455..1589817	-	363	WP_000985976.1	hypothetical protein	-
E3S74_RS07555	1589832..1590155	-	324	WP_000174994.1	hypothetical protein	-
E3S74_RS07560	1590234..1590395	-	162	WP_001285954.1	DUF1270 domain-containing protein	-
E3S74_RS07565	1590408..1590671	-	264	WP_001124160.1	helix-turn-helix domain-containing protein	-
E3S74_RS07570	1590696..1590911	-	216	WP_001097552.1	hypothetical protein	-
E3S74_RS07575	1590966..1591331	+	366	WP_001128433.1	hypothetical protein	-
E3S74_RS07580	1591300..1591545	-	246	WP_001025401.1	DUF2829 domain-containing protein	-
E3S74_RS07585	1591574..1592350	-	777	WP_001148544.1	Rha family transcriptional regulator	-
E3S74_RS07590	1592364..1592600	-	237	WP_001121027.1	helix-turn-helix domain-containing protein	-
E3S74_RS07595	1592752..1593066	+	315	WP_000333630.1	helix-turn-helix domain-containing protein	Antitoxin
E3S74_RS07600	1593079..1593540	+	462	WP_000525004.1	hypothetical protein	Toxin
E3S74_RS07605	1593558..1594142	+	585	WP_000825948.1	hypothetical protein	-
E3S74_RS07610	1594481..1595104	-	624	WP_001166482.1	hypothetical protein	-
E3S74_RS07615	1595214..1596419	+	1206	WP_000264191.1	site-specific integrase	-
E3S74_RS07620	1596462..1598504	-	2043	WP_001794062.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1551826..1614069	62243

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (4-62)

Sequences

Toxin

Protein

• Length: 154 a.a.
• Molecular weight: 18074.46 Da
• Isoelectric Point: 4.6915

>T148302 WP_000525004.1 NZ_CP047851:1593079-1593540 [Staphylococcus aureus]
MGLYEETLIQHDYIEIREADVLPDNLDGVWLGDLILIKRGLSDREKAGILFEELAHNKLTYGDIADYSKFNNRKFENYAR
RHGFISAVPLREIVEAYNYGVRNLYELSEYLQLSEEYILEAIEQYKKIYGIGTHYGEYSITFEPLRVFKYKEI

Nucleotide

• Length: 462 bp

>T148302 NZ_CP063489:17723-17971 [Escherichia coli]
ATGGGGAAAACAGATAAGCTACTGGCAAAGTTTTTAAACAGTAAAAAAACGTTTGAATGGGATGAACTGGTCGTTTTGTT
TTCCTCTTTGGGATATGTCAAAAAGGAAATGCAGGGGTCAAGAGTGCGGTTTTTCAATGCTGAAATCAATCACACCATAT
TAATGCATCGTCCACATCCGGAAAGTTATATCAAAGGTGGTACGCTGAAAGCAATTAAACAGAATCTGAAAGAGGCTGGA
TTATTATGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 12063.56 Da
• Isoelectric Point: 6.4736

>AT148302 WP_000333630.1 NZ_CP047851:1592752-1593066 [Staphylococcus aureus]
MCFSKRMKQSREKQGMTLAELGRKIGKTEATVQRYESGNIKNLKNDTIESIATALNVNPAYLMGWVEENDDEVQHRAAHL
EGELTDDEWQRVLDYADYIRSKRK

Nucleotide

• Length: 315 bp

>AT148302 NZ_CP063489:17968-18315 [Escherichia coli]
ATGAAACATTTAAAATATAAAGGATATTTAGGCACGGTTGAACCCGATTTTGAAAATAATGTCCTGTATGGAAAACTGGC
GTTTATTCGGGACCTGGTAACTTATGAGGCTTCTACATTAGCTGAACTGGAACAGGAGTTTAAAACATCTGTTGATCTGT
ATTTACAGTCTTGTGTGGAGGATGGAAAGGAACCTGATACCCCGTTTAAAGGCGTGTTTAACGTCAGGCTTGATCCAGAA
CTGCATCGTCGGGTAGCAGAAATGGCGATGGAAGAAGATTTATCGTTGAATGCCTTTGTTAATAAGGCTCTGGAAAAAGA
AGTCAGCAATCATCGCGCAGGGGCTTAA

Structures

Toxin

• AlphaFold DB: A0A2I7Y5B3

Antitoxin

• AlphaFold DB: A0A0E7YIA0