Detailed information of TA system TA148279

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2691800..2692329
• Replicon: chromosome
• Accession: NZ_CP047847
• Organism: Staphylococcus aureus strain UP_522

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: E3S80_RS13480
• Protein ID: WP_000621175.1
• Coordinates: 2691967..2692329 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: E3S80_RS13475
• Protein ID: WP_000948331.1
• Coordinates: 2691800..2691970 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3S80_RS13445	2686836..2687396	+	561	WP_001092410.1	K(+)-transporting ATPase subunit C	-
E3S80_RS13450	2687605..2688084	+	480	WP_031590204.1	hypothetical protein	-
E3S80_RS13455	2688077..2689660	+	1584	WP_001294626.1	PH domain-containing protein	-
E3S80_RS13460	2689647..2690138	+	492	WP_072353916.1	PH domain-containing protein	-
E3S80_RS13465	2690142..2690501	+	360	WP_000581197.1	holo-ACP synthase	-
E3S80_RS13470	2690567..2691715	+	1149	WP_001281154.1	alanine racemase	-
E3S80_RS13475	2691800..2691970	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
E3S80_RS13480	2691967..2692329	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
E3S80_RS13485	2692679..2693680	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
E3S80_RS13490	2693799..2694125	+	327	WP_001052491.1	anti-sigma factor antagonist	-
E3S80_RS13495	2694127..2694606	+	480	WP_001190829.1	anti-sigma B factor RsbW	-
E3S80_RS13500	2694581..2695351	+	771	WP_001041102.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T148279 WP_000621175.1 NZ_CP047847:2691967-2692329 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T148279 NZ_CP063486:21563-21979 [Escherichia coli]
GTGAACAAGACTTATATGCTTGATACCTGTATCTGCTCGTTCATCATGCGTGAACAGCCAGAAGCCGTCCTGAAGCGTCT
GGAGCAGGCGGTGCTACGCGGCCACCGTATAGTGGTCTCGGCCATCACCTACTCTGAAATGCGCTTCGGGGCTACCGGCC
CAAAGGCCTCACCACGTCACGTCCAACTGGTCGATGAATTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCC
GCAGTAGACGCCACCACGAAGATTAAAGTGGCACTGCGGCTGGCCGGGACGCCGATCGGCCCGAACGACACGGCGATTGC
CGGGCACGCCATCGCCGCCGGGGCGATACTGGTAACGAATAATACAAGAGAATTTGAGCGAGTGCCTGACCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT148279 WP_000948331.1 NZ_CP047847:2691800-2691970 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT148279 NZ_CP063486:21336-21566 [Escherichia coli]
ATGAGAACCGTATCGATATTCAAAAATGGCAACAACCGCGCCATCCGTCTGCCTCGTGATCTGGATTTTGAGGGGGTGAG
CGAGCTCGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGCCCCGTCCGGCCAACCTGGGGATCATTCCTAGAGTACG
AAAAAGCCGATCCAGATTTTATGGCGGAGCGCGAGGATGTTGTCAGCGACGAAGGGCGATTTAACCTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1