Detailed information of TA system TA148266

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tsbAT/-
• Location: 172023..172799
• Replicon: chromosome
• Accession: NZ_CP047847
• Organism: Staphylococcus aureus strain UP_522

Toxin (Protein)

• Gene name: tsbT
• Uniprot ID: X5E2E6
• Locus tag: E3S80_RS01150
• Protein ID: WP_000031108.1
• Coordinates: 172647..172799 (+)
• Length: 51 a.a.

Antitoxin (Protein)

• Gene name: tsbA
• Uniprot ID: -
• Locus tag: E3S80_RS01145
• Protein ID: WP_001251230.1
• Coordinates: 172023..172622 (+)
• Length: 200 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3S80_RS01125	167939..169396	+	1458	WP_000649908.1	ABC transporter permease subunit	-
E3S80_RS01130	169389..170111	+	723	WP_047928685.1	amino acid ABC transporter ATP-binding protein	-
E3S80_RS01135	170262..171389	+	1128	WP_000379988.1	tRNA epoxyqueuosine(34) reductase QueG	-
E3S80_RS01140	171394..171864	+	471	WP_000181394.1	tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL	-
E3S80_RS01145	172023..172622	+	600	WP_001251230.1	hypothetical protein	Antitoxin
E3S80_RS01150	172647..172799	+	153	WP_000031108.1	hypothetical protein	Toxin
E3S80_RS01155	173635..174030	+	396	WP_000901023.1	hypothetical protein	-
E3S80_RS01160	174225..175610	+	1386	WP_000116229.1	class II fumarate hydratase	-
E3S80_RS01165	176062..176883	-	822	WP_000669376.1	RluA family pseudouridine synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Glucosamine-6-phosphate isomerase (124-196)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5936.28 Da
• Isoelectric Point: 3.8962

>T148266 WP_000031108.1 NZ_CP047847:172647-172799 [Staphylococcus aureus]
MSKDKDPKLNYHEEENSMVTDFEDLKELGKEMEQISDQNDQEKNSEEDSQ

Nucleotide

• Length: 153 bp

>T148266 NZ_CP063483:2727874-2727977 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 200 a.a.
• Molecular weight: 22369.51 Da
• Isoelectric Point: 4.9728

>AT148266 WP_001251230.1 NZ_CP047847:172023-172622 [Staphylococcus aureus]
MAMNFKVFDNSQLVAEYAADIIRKQFNNNPTTIAGFHLDTDQAPVLDELKKNVEKYAVDFSQINILDYDDKKSYFEALGV
PAGQVYPIAYEKDAIELIADKIKTKENKGKLTLQVVSIDEQGKLNVSIRQGLMEAREIFLVVTGANKRDVVEKLYQENGK
TSFEPADLKAHRMVNVILDKEAAAGLPEDVKAYFTSRFA

Nucleotide

• Length: 600 bp

>AT148266 NZ_CP063483:c2728015-2727744 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Structures

Toxin

• AlphaFold DB: X5E2E6