Detailed information of TA system TA148262

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TscAT/-
• Location: 2708254..2708783
• Replicon: chromosome
• Accession: NZ_CP047845
• Organism: Staphylococcus aureus strain UP_591

Toxin (Protein)

• Gene name: TscT
• Uniprot ID: K7ZRX6
• Locus tag: E3S82_RS13270
• Protein ID: WP_001103939.1
• Coordinates: 2708466..2708783 (+)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: TscA
• Uniprot ID: X5IY59
• Locus tag: E3S82_RS13265
• Protein ID: WP_001058494.1
• Coordinates: 2708254..2708463 (+)
• Length: 70 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3S82_RS13230	2704584..2705048	+	465	WP_001085185.1	SsrA-binding protein SmpB	-
E3S82_RS13240	2705611..2706717	-	1107	WP_000149511.1	site-specific integrase	-
E3S82_RS13245	2706707..2707510	-	804	WP_000358990.1	helix-turn-helix domain-containing protein	-
E3S82_RS13250	2707646..2707849	+	204	WP_001045296.1	transcriptional regulator	-
E3S82_RS13255	2707885..2708103	+	219	WP_000163544.1	helix-turn-helix domain-containing protein	-
E3S82_RS13260	2708115..2708261	+	147	WP_000784885.1	hypothetical protein	-
E3S82_RS13265	2708254..2708463	+	210	WP_001058494.1	hypothetical protein	Antitoxin
E3S82_RS13270	2708466..2708783	+	318	WP_001103939.1	DUF1474 family protein	Toxin
E3S82_RS13275	2708847..2709716	+	870	WP_001002717.1	primase alpha helix C-terminal domain-containing protein	-
E3S82_RS13280	2709733..2711190	+	1458	WP_000390453.1	virulence-associated E family protein	-
E3S82_RS13285	2711491..2711853	+	363	WP_001039170.1	hypothetical protein	-
E3S82_RS13290	2711855..2712139	+	285	WP_000998185.1	hypothetical protein	-
E3S82_RS13295	2712136..2712777	+	642	WP_001019808.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Protein of unknown function DUF1474 (1-98)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12547.17 Da
• Isoelectric Point: 5.0161

>T148262 WP_001103939.1 NZ_CP047845:2708466-2708783 [Staphylococcus aureus]
MNWEIKDLMCDIEVIKQKINDVATKHAWFVEDRFVKNELETKREHINFSASYLEHRIQNEHTVELLHVYLKEFGELIQKF
HEIEKASSENFGEVSDDAQKLKITE

Nucleotide

• Length: 318 bp

>T148262 NZ_CP063483:1690980-1691315 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 70 a.a.
• Molecular weight: 8268.31 Da
• Isoelectric Point: 4.3718

>AT148262 WP_001058494.1 NZ_CP047845:2708254-2708463 [Staphylococcus aureus]
MNNEQKEVIEHVVYQLELSVMNNLESYEHTEYVNSIEVVSEISREKHLELIMKWCAQELKNNFQLEKGE

Nucleotide

• Length: 210 bp

>AT148262 NZ_CP063483:1690732-1690980 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Toxin

• AlphaFold DB: K7ZRX6

Antitoxin

• AlphaFold DB: X5IY59