Detailed information of TA system TA148258

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1232342..1232871
• Replicon: chromosome
• Accession: NZ_CP047845
• Organism: Staphylococcus aureus strain UP_591

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: E3S82_RS06185
• Protein ID: WP_000621175.1
• Coordinates: 1232342..1232704 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: E3S82_RS06190
• Protein ID: WP_000948331.1
• Coordinates: 1232701..1232871 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3S82_RS06165	1229321..1230091	-	771	WP_001041107.1	RNA polymerase sigma factor SigB	-
E3S82_RS06170	1230066..1230545	-	480	WP_001190825.1	anti-sigma B factor RsbW	-
E3S82_RS06175	1230547..1230873	-	327	WP_001052491.1	anti-sigma factor antagonist	-
E3S82_RS06180	1230992..1231993	-	1002	WP_031928242.1	PP2C family protein-serine/threonine phosphatase	-
E3S82_RS06185	1232342..1232704	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
E3S82_RS06190	1232701..1232871	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
E3S82_RS06195	1232956..1234104	-	1149	WP_001281140.1	alanine racemase	-
E3S82_RS06200	1234170..1234529	-	360	WP_000581199.1	holo-ACP synthase	-
E3S82_RS06205	1234533..1235024	-	492	WP_001205907.1	PH domain-containing protein	-
E3S82_RS06210	1235011..1236594	-	1584	WP_001294637.1	PH domain-containing protein	-
E3S82_RS06215	1236587..1237066	-	480	WP_001287077.1	hypothetical protein	-
E3S82_RS06220	1237274..1237834	-	561	WP_001092406.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T148258 WP_000621175.1 NZ_CP047845:c1232704-1232342 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T148258 NZ_CP063483:c1334811-1334347 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGTTCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT148258 WP_000948331.1 NZ_CP047845:c1232871-1232701 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT148258 NZ_CP063483:c1335146-1334811 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1