Detailed information of TA system TA148214

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsaAT/-
• Location: 1222011..1222488
• Replicon: chromosome
• Accession: NZ_CP047839
• Organism: Staphylococcus aureus strain UP_678

Toxin (Protein)

• Gene name: TsaT
• Uniprot ID: T1Y7S2
• Locus tag: E3S85_RS06130
• Protein ID: WP_000052076.1
• Coordinates: 1222300..1222488 (+)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: TsaA
• Uniprot ID: T1Y6P8
• Locus tag: E3S85_RS06125
• Protein ID: WP_001059690.1
• Coordinates: 1222011..1222271 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3S85_RS06090	1217403..1218119	-	717	WP_160176027.1	type I 3-dehydroquinate dehydratase	-
E3S85_RS06095	1218267..1218689	+	423	WP_000974455.1	organic hydroperoxide resistance protein	-
E3S85_RS06100	1219088..1219582	+	495	WP_000569884.1	GNAT family N-acetyltransferase	-
E3S85_RS06105	1219738..1220355	+	618	WP_000255548.1	LysE/ArgO family amino acid transporter	-
E3S85_RS06110	1220428..1221009	-	582	WP_001140696.1	phosphoglycerate mutase family protein	-
E3S85_RS06115	1221234..1221470	+	237	WP_000368687.1	hypothetical protein	-
E3S85_RS06120	1221467..1221670	-	204	WP_000145163.1	sterile alpha motif-like domain-containing protein	-
E3S85_RS06125	1222011..1222271	+	261	WP_001059690.1	hypothetical protein	Antitoxin
E3S85_RS06130	1222300..1222488	+	189	WP_000052076.1	hypothetical protein	Toxin
E3S85_RS06135	1222676..1223245	+	570	WP_001106663.1	hypothetical protein	-
E3S85_RS06140	1223333..1223617	+	285	WP_001004401.1	hypothetical protein	-
E3S85_RS06145	1223679..1223897	+	219	WP_000005879.1	hypothetical protein	-
E3S85_RS06150	1224394..1224594	-	201	WP_001059082.1	cold-shock protein	-
E3S85_RS06155	1224951..1225640	-	690	WP_160176028.1	thermonuclease family protein	-
E3S85_RS06160	1225981..1226538	-	558	WP_000824491.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	vWbp / clpP	1211676..1343042	131366

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7142.82 Da
• Isoelectric Point: 9.9971

>T148214 WP_000052076.1 NZ_CP047839:1222300-1222488 [Staphylococcus aureus]
MSLHFAILFWLALIFLVAATFILVLMKKTGKESKKESYLSFTVILYIFGFAILIYTFIFGVL

Nucleotide

• Length: 189 bp

>T148214 NZ_CP063476:1944510-1944896 [Escherichia coli]
ATGGGTGTGTATAAGGCAAGACGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAGGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGTTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGTAGTAAAGGAACCAAAGAAATCGAAGATGATGAACTGGCGGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAGAGGATTTGATCGAAACTGGCTTTTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9940.09 Da
• Isoelectric Point: 4.0624

>AT148214 WP_001059690.1 NZ_CP047839:1222011-1222271 [Staphylococcus aureus]
MNNILLNAINIVITTTFVIFNILITYNKDLDDLCWLLPGIIICGVILIVSFTIAMITKNWLSEILFFINIVLVLYYIYPI
FYSFIG

Nucleotide

• Length: 261 bp

>AT148214 NZ_CP063476:1944886-1945215 [Escherichia coli]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCACTGGGCCTGATGTCTGATAGTGAAGTTGA
TGCCATTGCCGTCATGGTCAAAGCTCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGTAATGAAATAAAACCGAGCGGCCCGGCATTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A0E0VP18

Antitoxin

• AlphaFold DB: A0A0E0VN56