Detailed information of TA system TA148200

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2689572..2690101
• Replicon: chromosome
• Accession: NZ_CP047837
• Organism: Staphylococcus aureus strain UP_719

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: E3S86_RS13260
• Protein ID: WP_000621175.1
• Coordinates: 2689739..2690101 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: E3S86_RS13255
• Protein ID: WP_000948331.1
• Coordinates: 2689572..2689742 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3S86_RS13225	2684609..2685169	+	561	WP_001092406.1	K(+)-transporting ATPase subunit C	-
E3S86_RS13230	2685377..2685856	+	480	WP_001287077.1	hypothetical protein	-
E3S86_RS13235	2685849..2687432	+	1584	WP_001294637.1	PH domain-containing protein	-
E3S86_RS13240	2687419..2687910	+	492	WP_001205907.1	PH domain-containing protein	-
E3S86_RS13245	2687914..2688273	+	360	WP_000581199.1	holo-ACP synthase	-
E3S86_RS13250	2688339..2689487	+	1149	WP_001281140.1	alanine racemase	-
E3S86_RS13255	2689572..2689742	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
E3S86_RS13260	2689739..2690101	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
E3S86_RS13265	2690450..2691451	+	1002	WP_031928242.1	PP2C family protein-serine/threonine phosphatase	-
E3S86_RS13270	2691570..2691896	+	327	WP_001052491.1	anti-sigma factor antagonist	-
E3S86_RS13275	2691898..2692377	+	480	WP_001190825.1	anti-sigma B factor RsbW	-
E3S86_RS13280	2692352..2693122	+	771	WP_001041107.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T148200 WP_000621175.1 NZ_CP047837:2689739-2690101 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T148200 NZ_CP063473:c3807921-3807625 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT148200 WP_000948331.1 NZ_CP047837:2689572-2689742 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT148200 NZ_CP063473:c3807623-3807228 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1