Detailed information of TA system TA148196

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TscAT/-
• Location: 1210253..1210782
• Replicon: chromosome
• Accession: NZ_CP047837
• Organism: Staphylococcus aureus strain UP_719

Toxin (Protein)

• Gene name: TscT
• Uniprot ID: K7ZRX6
• Locus tag: E3S86_RS06145
• Protein ID: WP_001103939.1
• Coordinates: 1210253..1210570 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: TscA
• Uniprot ID: X5IY59
• Locus tag: E3S86_RS06150
• Protein ID: WP_001058494.1
• Coordinates: 1210573..1210782 (-)
• Length: 70 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3S86_RS06120	1206259..1206900	-	642	WP_001019808.1	hypothetical protein	-
E3S86_RS06125	1206897..1207181	-	285	WP_000998185.1	hypothetical protein	-
E3S86_RS06130	1207183..1207545	-	363	WP_001039170.1	hypothetical protein	-
E3S86_RS06135	1207846..1209303	-	1458	WP_000390453.1	virulence-associated E family protein	-
E3S86_RS06140	1209320..1210189	-	870	WP_001002717.1	primase alpha helix C-terminal domain-containing protein	-
E3S86_RS06145	1210253..1210570	-	318	WP_001103939.1	DUF1474 family protein	Toxin
E3S86_RS06150	1210573..1210782	-	210	WP_001058494.1	hypothetical protein	Antitoxin
E3S86_RS06155	1210775..1210921	-	147	WP_000784885.1	hypothetical protein	-
E3S86_RS06160	1210933..1211151	-	219	WP_000163544.1	helix-turn-helix domain-containing protein	-
E3S86_RS06165	1211187..1211390	-	204	WP_001045296.1	transcriptional regulator	-
E3S86_RS06170	1211526..1212329	+	804	WP_000358990.1	helix-turn-helix domain-containing protein	-
E3S86_RS06175	1212319..1213425	+	1107	WP_000149511.1	site-specific integrase	-
E3S86_RS06185	1213988..1214452	-	465	WP_001085185.1	SsrA-binding protein SmpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	vWbp / sell / sec	1170670..1216846	46176

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Protein of unknown function DUF1474 (1-98)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12547.17 Da
• Isoelectric Point: 5.0161

>T148196 WP_001103939.1 NZ_CP047837:c1210570-1210253 [Staphylococcus aureus]
MNWEIKDLMCDIEVIKQKINDVATKHAWFVEDRFVKNELETKREHINFSASYLEHRIQNEHTVELLHVYLKEFGELIQKF
HEIEKASSENFGEVSDDAQKLKITE

Nucleotide

• Length: 318 bp

>T148196 NZ_CP063473:c2779290-2779036 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCGCTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 70 a.a.
• Molecular weight: 8268.31 Da
• Isoelectric Point: 4.3718

>AT148196 WP_001058494.1 NZ_CP047837:c1210782-1210573 [Staphylococcus aureus]
MNNEQKEVIEHVVYQLELSVMNNLESYEHTEYVNSIEVVSEISREKHLELIMKWCAQELKNNFQLEKGE

Nucleotide

• Length: 210 bp

>AT148196 NZ_CP063473:c2779538-2779287 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Structures

Toxin

• AlphaFold DB: K7ZRX6

Antitoxin

• AlphaFold DB: X5IY59