Detailed information of TA system TA148189

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2698311..2698840
• Replicon: chromosome
• Accession: NZ_CP047835
• Organism: Staphylococcus aureus strain UP_883

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: E3S91_RS13270
• Protein ID: WP_000621175.1
• Coordinates: 2698478..2698840 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: E3S91_RS13265
• Protein ID: WP_000948331.1
• Coordinates: 2698311..2698481 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3S91_RS13235	2693347..2693907	+	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-
E3S91_RS13240	2694116..2694595	+	480	WP_001287088.1	hypothetical protein	-
E3S91_RS13245	2694588..2696171	+	1584	WP_001294626.1	PH domain-containing protein	-
E3S91_RS13250	2696158..2696649	+	492	WP_001205910.1	PH domain-containing protein	-
E3S91_RS13255	2696653..2697012	+	360	WP_000581200.1	holo-ACP synthase	-
E3S91_RS13260	2697078..2698226	+	1149	WP_001281145.1	alanine racemase	-
E3S91_RS13265	2698311..2698481	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
E3S91_RS13270	2698478..2698840	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
E3S91_RS13275	2699190..2700191	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
E3S91_RS13280	2700310..2700636	+	327	WP_001052491.1	anti-sigma factor antagonist	-
E3S91_RS13285	2700638..2701117	+	480	WP_001190830.1	anti-sigma B factor RsbW	-
E3S91_RS13290	2701092..2701862	+	771	WP_064128499.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T148189 WP_000621175.1 NZ_CP047835:2698478-2698840 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T148189 NZ_CP063473:c1545838-1545452 [Escherichia coli]
ATGGGTGTGTATAAGGCAAGGCGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAAGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGGTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGCAGTAAAGGAACCAAAGAAATCGAAGATGATGAACTGGCAGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAGAGGATTTGATCGAAACTGGCTTCTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT148189 WP_000948331.1 NZ_CP047835:2698311-2698481 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT148189 NZ_CP063473:c1545462-1545133 [Escherichia coli]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCATTGGGCCTGATGTCTGATAGTGAGGTTGA
TGCCATTGCCGTCATGGTCAAAGCCCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGTAATGAAATAAAACCGAGCGGCCCGGCACTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1