Detailed information of TA system TA148172

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2701255..2701784
• Replicon: chromosome
• Accession: NZ_CP047833
• Organism: Staphylococcus aureus strain UP_926

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: E3S92_RS13310
• Protein ID: WP_000621175.1
• Coordinates: 2701422..2701784 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: E3S92_RS13305
• Protein ID: WP_000948331.1
• Coordinates: 2701255..2701425 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3S92_RS13275	2696292..2696852	+	561	WP_001092406.1	K(+)-transporting ATPase subunit C	-
E3S92_RS13280	2697060..2697539	+	480	WP_001287077.1	hypothetical protein	-
E3S92_RS13285	2697532..2699115	+	1584	WP_001294637.1	PH domain-containing protein	-
E3S92_RS13290	2699102..2699593	+	492	WP_001205907.1	PH domain-containing protein	-
E3S92_RS13295	2699597..2699956	+	360	WP_000581199.1	holo-ACP synthase	-
E3S92_RS13300	2700022..2701170	+	1149	WP_001281140.1	alanine racemase	-
E3S92_RS13305	2701255..2701425	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
E3S92_RS13310	2701422..2701784	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
E3S92_RS13315	2702133..2703134	+	1002	WP_042744356.1	PP2C family protein-serine/threonine phosphatase	-
E3S92_RS13320	2703253..2703579	+	327	WP_001052491.1	anti-sigma factor antagonist	-
E3S92_RS13325	2703581..2704060	+	480	WP_001190825.1	anti-sigma B factor RsbW	-
E3S92_RS13330	2704035..2704805	+	771	WP_001041107.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T148172 WP_000621175.1 NZ_CP047833:2701422-2701784 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T148172 NZ_CP063467:c2401530-2401132 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT148172 WP_000948331.1 NZ_CP047833:2701255-2701425 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT148172 NZ_CP063467:c2401826-2401533 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1