Detailed information of TA system TA148157

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2047450..2047979
• Replicon: chromosome
• Accession: NZ_CP047830
• Organism: Staphylococcus aureus strain UP_996

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: E3S94_RS10070
• Protein ID: WP_000621175.1
• Coordinates: 2047450..2047812 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: E3S94_RS10075
• Protein ID: WP_000948331.1
• Coordinates: 2047809..2047979 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3S94_RS10050	2044428..2045198	-	771	WP_001041103.1	RNA polymerase sigma factor SigB	-
E3S94_RS10055	2045173..2045652	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
E3S94_RS10060	2045654..2045980	-	327	WP_001052491.1	anti-sigma factor antagonist	-
E3S94_RS10065	2046099..2047100	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
E3S94_RS10070	2047450..2047812	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
E3S94_RS10075	2047809..2047979	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
E3S94_RS10080	2048064..2049212	-	1149	WP_001281145.1	alanine racemase	-
E3S94_RS10085	2049278..2049637	-	360	WP_000581200.1	holo-ACP synthase	-
E3S94_RS10090	2049641..2050132	-	492	WP_001205910.1	PH domain-containing protein	-
E3S94_RS10095	2050119..2051702	-	1584	WP_001294626.1	PH domain-containing protein	-
E3S94_RS10100	2051695..2052174	-	480	WP_001287088.1	hypothetical protein	-
E3S94_RS10105	2052382..2052942	-	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T148157 WP_000621175.1 NZ_CP047830:c2047812-2047450 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T148157 NZ_CP063462:c84825-84409 [Escherichia coli]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAGCCTGAAGCAGTGCTTAAGAATCT
GGAGCAGGCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGCGCCACCGGTC
CAAAGGCATCGCCACGCCACGTGCAGCTGGTTGACGCGTTCTGCGCCCGTCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGATGCAACCACGGAGGTTAAGGTGGCGCTGCGCCTCGCCGGCACGCCGATCGGTCCAAACGACACGGCGATCGC
CGGGCACGCCATCGCCACCGGGGCAATACTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTGCCTGGACTGGTACTGG
AAGACTGGGCAGGATAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT148157 WP_000948331.1 NZ_CP047830:c2047979-2047809 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT148157 NZ_CP063462:c85052-84822 [Escherichia coli]
ATGAGAACCGTATCTATTTTCAAAAATGGCAATAACCGCGCGATCCGTCTGCCCCGCGATCTGGATTTTGAGGGGGTGAG
TGAGCTGGAGATTGTACGGGAAGGGGACAGAATCATTCTGCGTCCCCTCCGCCCGAGCTGGAGCTCATTCCTGGAGCTAG
AAAAGGCCGATCCGGGCTTTATGACGGAACGCGAGGACGTTGTCTGCGACGAAGGACGATTTAACCTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1