Detailed information of TA system TA148142

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2668564..2669093
• Replicon: chromosome
• Accession: NZ_CP047826
• Organism: Staphylococcus aureus strain UP_1239

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: E3T01_RS13105
• Protein ID: WP_000621175.1
• Coordinates: 2668731..2669093 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: E3T01_RS13100
• Protein ID: WP_000948331.1
• Coordinates: 2668564..2668734 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3T01_RS13070	2663600..2664160	+	561	WP_001092416.1	K(+)-transporting ATPase subunit C	-
E3T01_RS13075	2664369..2664848	+	480	WP_001287090.1	hypothetical protein	-
E3T01_RS13080	2664841..2666424	+	1584	WP_001294626.1	PH domain-containing protein	-
E3T01_RS13085	2666411..2666902	+	492	WP_001205910.1	PH domain-containing protein	-
E3T01_RS13090	2666906..2667265	+	360	WP_000581200.1	holo-ACP synthase	-
E3T01_RS13095	2667331..2668479	+	1149	WP_001281151.1	alanine racemase	-
E3T01_RS13100	2668564..2668734	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
E3T01_RS13105	2668731..2669093	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
E3T01_RS13110	2669442..2670443	+	1002	WP_061839378.1	PP2C family protein-serine/threonine phosphatase	-
E3T01_RS13115	2670562..2670888	+	327	WP_001052491.1	anti-sigma factor antagonist	-
E3T01_RS13120	2670890..2671369	+	480	WP_001190829.1	anti-sigma B factor RsbW	-
E3T01_RS13125	2671344..2672114	+	771	WP_001041102.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T148142 WP_000621175.1 NZ_CP047826:2668731-2669093 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T148142 NZ_CP063459:c1889437-1889030 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT148142 WP_000948331.1 NZ_CP047826:2668564-2668734 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT148142 NZ_CP063459:c1889684-1889418 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1