Detailed information of TA system TA148131

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tsbAT/-
• Location: 120646..121422
• Replicon: chromosome
• Accession: NZ_CP047826
• Organism: Staphylococcus aureus strain UP_1239

Toxin (Protein)

• Gene name: tsbT
• Uniprot ID: X5E2E6
• Locus tag: E3T01_RS00770
• Protein ID: WP_000031108.1
• Coordinates: 121270..121422 (+)
• Length: 51 a.a.

Antitoxin (Protein)

• Gene name: tsbA
• Uniprot ID: W8U4V4
• Locus tag: E3T01_RS00765
• Protein ID: WP_001251224.1
• Coordinates: 120646..121245 (+)
• Length: 200 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3T01_RS00745	116562..118019	+	1458	WP_000649907.1	ABC transporter permease subunit	-
E3T01_RS00750	118012..118734	+	723	WP_000590809.1	amino acid ABC transporter ATP-binding protein	-
E3T01_RS00755	118885..120012	+	1128	WP_000379978.1	tRNA epoxyqueuosine(34) reductase QueG	-
E3T01_RS00760	120017..120487	+	471	WP_000181394.1	tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL	-
E3T01_RS00765	120646..121245	+	600	WP_001251224.1	glucosamine-6-phosphate isomerase	Antitoxin
E3T01_RS00770	121270..121422	+	153	WP_000031108.1	hypothetical protein	Toxin
E3T01_RS00775	121968..122363	+	396	WP_000901023.1	hypothetical protein	-
E3T01_RS00780	122559..123944	+	1386	WP_000116230.1	class II fumarate hydratase	-
E3T01_RS00785	124397..125218	-	822	WP_000669373.1	RluA family pseudouridine synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Glucosamine-6-phosphate isomerase (124-196)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5936.28 Da
• Isoelectric Point: 3.8962

>T148131 WP_000031108.1 NZ_CP047826:121270-121422 [Staphylococcus aureus]
MSKDKDPKLNYHEEENSMVTDFEDLKELGKEMEQISDQNDQEKNSEEDSQ

Nucleotide

• Length: 153 bp

>T148131 NZ_CP063455:4574840-4575190 [Pseudomonas protegens]
ATGAATGCTCTGTTTATTGAACTACCGGCATTTGCACGATACCGAAAGGACTTCCTTGACGACGAGTTGTTCTGCGAACT
GCAGATCGAGTTGCTGCGCCACCCGCACGCGGGGGTTCTGATTCAGGGCACTGGCGGCTTGCGCAAGATGCGTTTCGTCG
ATGAGCGACGCAACAAGGGCAGGCGTGGCGGGCTGAGAGTGATTTATTACTGGTGGTCCGGAGGGGCGCAGTTCTGGCTC
TTCACCCTGTTCGACAAGGAGCAGATGGACGACCTGCTACCGCAGCACCGACAACAGCTCAAACAACTGCTGGAACGTGA
AATCGAGGCCAGAAGACATGATCAAACGTGA

Antitoxin

Protein

• Length: 200 a.a.
• Molecular weight: 22343.47 Da
• Isoelectric Point: 5.1445

>AT148131 WP_001251224.1 NZ_CP047826:120646-121245 [Staphylococcus aureus]
MAMNFKVFDNSQLVAEYAADIIRKQFNNNPTTIAGFHLDTDQAPVLDELKKNVEKHAVDFSQINILDYDDKKSYFEALGV
PAGQVYPIAYEKDAIELIADKIKTKENKGKLTLQVVSIDEQGKLNVSIRQGLMEAREIFLVVTGANKRDVVEKLYQENGK
TSFEPADLKAHRMVNVILDKEAAAGLPEDVKAYFTSRFA

Nucleotide

• Length: 600 bp

>AT148131 NZ_CP063455:4575180-4575494 [Pseudomonas protegens]
ATCAAACGTGACATCTTTTCCGAACTGGTAGAAGGCATCGACGCCCTCACCCAGGAGCGCCAGGGCAAGATCACCCTGCG
CAGCCATAAAGTGAAGCTGGACAAACTGCCCCCCATCACTGCTACCGAGGTGATGAACATCCGCCAGCAACTCAACCTCT
CGCGTTCAGTATTCGCCATGTACCTGCGCACCAACACCCGTACCCTGGAAAACTGGGAACAAGGCCGCGCCACACCCAAC
GCCCAGGCCACGACCTTGATGCGCCTGGTGGAGCGCTACCCGGACACAGTGGAGCGCCTCGCCTCGCTGGCCTGA

Structures

Toxin

• AlphaFold DB: X5E2E6

Antitoxin

• AlphaFold DB: W8U4V4