Detailed information of TA system TA148127

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2738473..2739002
• Replicon: chromosome
• Accession: NZ_CP047822
• Organism: Staphylococcus aureus strain UP_1278

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: E3T02_RS13550
• Protein ID: WP_000621175.1
• Coordinates: 2738640..2739002 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: E3T02_RS13545
• Protein ID: WP_000948331.1
• Coordinates: 2738473..2738643 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3T02_RS13515	2733509..2734069	+	561	WP_001092409.1	K(+)-transporting ATPase subunit C	-
E3T02_RS13520	2734278..2734757	+	480	WP_160194456.1	hypothetical protein	-
E3T02_RS13525	2734750..2736333	+	1584	WP_045173493.1	PH domain-containing protein	-
E3T02_RS13530	2736320..2736811	+	492	WP_078103494.1	PH domain-containing protein	-
E3T02_RS13535	2736815..2737174	+	360	WP_000581200.1	holo-ACP synthase	-
E3T02_RS13540	2737240..2738388	+	1149	WP_001281145.1	alanine racemase	-
E3T02_RS13545	2738473..2738643	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
E3T02_RS13550	2738640..2739002	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
E3T02_RS13555	2739351..2740352	+	1002	WP_160194457.1	PP2C family protein-serine/threonine phosphatase	-
E3T02_RS13560	2740471..2740797	+	327	WP_001052491.1	anti-sigma factor antagonist	-
E3T02_RS13565	2740799..2741278	+	480	WP_001190829.1	anti-sigma B factor RsbW	-
E3T02_RS13570	2741253..2742023	+	771	WP_001041103.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T148127 WP_000621175.1 NZ_CP047822:2738640-2739002 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T148127 NZ_CP063455:c1880103-1879804 [Pseudomonas protegens]
ATGAAATATCTCATCCAACAAACCCAGTCCTTTGCCAGATGGCACTCATCACTGAAGGACTTGCGCGCAAAAATCTCCAT
TGCCCGCCGCATCGATCGCGCTGCAACAGGCAATCTGGGGGATGTGAAATCCGTGGGAGAAGGTATTTCGGAACTGCGCG
TGGACGTGGGTCCCGGGTATCGGCTGTACTTCACCCAGCGTGGCGGCGTGATCATTGTGTTGCTGGCAGGCGGGGACAAA
TCCTCTCAAGACGCGGACATTCGCCGGGCAAAGATATTGGCAAAGGACCTTTCAACATGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT148127 WP_000948331.1 NZ_CP047822:2738473-2738643 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT148127 NZ_CP063455:c1879807-1879499 [Pseudomonas protegens]
ATGACCCAGATATTGACCCACTTCGACATGGCCGAGTTGCTGGACAGCGACCAGGCCATCGCAGAGTACCTGTCACAAGT
GCTGGCCGACGGTGATACCGAGGAGTTTCTTCGAGCCATCGGCTACGTAGCCAAAGCTCGGGGTATGTCCCAGATTGCCC
GGGATGCCGGGATGGGCCGGGAAAGCCTGTACAAGGCTTTTGCCCCGGGCTCAAAACCGCGATTCGACACCGTCTTGAAG
GTCATTCATGCCTTGGGGATCGATCTGCAGGCCCAGCCAGGGCATGTGGAGAAACCCAAGGCCAGTTGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1