Detailed information of TA system TA148095

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2667001..2667530
• Replicon: chromosome
• Accession: NZ_CP047817
• Organism: Staphylococcus aureus strain UP_1433

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: E3T06_RS13100
• Protein ID: WP_000621175.1
• Coordinates: 2667168..2667530 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: E3T06_RS13095
• Protein ID: WP_000948331.1
• Coordinates: 2667001..2667171 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3T06_RS13065	2662038..2662598	+	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-
E3T06_RS13070	2662806..2663285	+	480	WP_001287087.1	hypothetical protein	-
E3T06_RS13075	2663278..2664861	+	1584	WP_001294626.1	PH domain-containing protein	-
E3T06_RS13080	2664848..2665339	+	492	WP_001205912.1	PH domain-containing protein	-
E3T06_RS13085	2665343..2665702	+	360	WP_000581197.1	holo-ACP synthase	-
E3T06_RS13090	2665768..2666916	+	1149	WP_001281154.1	alanine racemase	-
E3T06_RS13095	2667001..2667171	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
E3T06_RS13100	2667168..2667530	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
E3T06_RS13105	2667880..2668881	+	1002	WP_061739989.1	PP2C family protein-serine/threonine phosphatase	-
E3T06_RS13110	2669000..2669326	+	327	WP_001052491.1	anti-sigma factor antagonist	-
E3T06_RS13115	2669328..2669807	+	480	WP_001190829.1	anti-sigma B factor RsbW	-
E3T06_RS13120	2669782..2670552	+	771	WP_001041111.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T148095 WP_000621175.1 NZ_CP047817:2667168-2667530 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T148095 NZ_CP063427:c4422177-4421761 [Citrobacter sp. BDA59-3]
GTGGTCCTGTGGCAATCTGATATACGCGTCTCATGGCGCTCACAGTGGCTCTCACTTTTACTCCATGGCTTGATGGCGGC
TTTCGTGTTGCTGTTACCCTGGCCATTGAGCTATACGCCGCTTTGGTTACTGCTTTTGTCACTGGTGGTGTTCGATAGCG
TACGGAGTCAGCGCCGCATTAATGCCTGTCAGGGAGAGATCAAACTGCTGATGGATGCACGTTTGCGCTGGCAAGGCGTG
GAGTGGGAAATCGTCGGTACACCGTGGATGCTGAATATTGGCATGATTCTGCGTTTGCGTCGGGAAAAAGATGGTCGCCG
ACAGCATCTGTGGCTGGCCGCAGATAGCATGGATGCGCAAGAGTGGCGTGATTTGCGCCGACTGATTTCGCAACAATCGG
CGCAGCGTCAGCACTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT148095 WP_000948331.1 NZ_CP047817:2667001-2667171 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT148095 NZ_CP063427:c4422424-4422158 [Citrobacter sp. BDA59-3]
ATGGATATTAACAACAAAGCCCGTATTCATTGGGCATGTCGGCGCGGAATGCGTGAGCTGGATATTTCCATCATGCCTTT
CTTTGAACATGAATATGATTCGCTGAGTGATGATGACAAACATGTGTTTGTGCGTCTTCTGCAGAGCGATGACCCGGATT
TATTTAACTGGCTGATGAATCACGGCAAACCTCAGGACGAGGCGTTGCAGCGAATGATCCAGTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1