Detailed information of TA system TA148070

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tsbAT/-
• Location: 1881508..1882284
• Replicon: chromosome
• Accession: NZ_CP047815
• Organism: Staphylococcus aureus strain UP_1484

Toxin (Protein)

• Gene name: tsbT
• Uniprot ID: X5E2E6
• Locus tag: E3T08_RS09000
• Protein ID: WP_000031108.1
• Coordinates: 1881508..1881660 (-)
• Length: 51 a.a.

Antitoxin (Protein)

• Gene name: tsbA
• Uniprot ID: W8U4V4
• Locus tag: E3T08_RS09005
• Protein ID: WP_001251224.1
• Coordinates: 1881685..1882284 (-)
• Length: 200 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3T08_RS08985	1877123..1877944	+	822	WP_000669376.1	RluA family pseudouridine synthase	-
E3T08_RS08990	1878405..1879790	-	1386	WP_000116224.1	class II fumarate hydratase	-
E3T08_RS08995	1879986..1880381	-	396	WP_000901021.1	hypothetical protein	-
E3T08_RS09000	1881508..1881660	-	153	WP_000031108.1	hypothetical protein	Toxin
E3T08_RS09005	1881685..1882284	-	600	WP_001251224.1	glucosamine-6-phosphate isomerase	Antitoxin
E3T08_RS09010	1882442..1882912	-	471	WP_000181394.1	tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL	-
E3T08_RS09015	1882917..1884044	-	1128	WP_000379985.1	tRNA epoxyqueuosine(34) reductase QueG	-
E3T08_RS09020	1884195..1884917	-	723	WP_000590809.1	amino acid ABC transporter ATP-binding protein	-
E3T08_RS09025	1884910..1886367	-	1458	WP_000649908.1	ABC transporter permease subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Glucosamine-6-phosphate isomerase (124-196)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5936.28 Da
• Isoelectric Point: 3.8962

>T148070 WP_000031108.1 NZ_CP047815:c1881660-1881508 [Staphylococcus aureus]
MSKDKDPKLNYHEEENSMVTDFEDLKELGKEMEQISDQNDQEKNSEEDSQ

Nucleotide

• Length: 153 bp

>T148070 NZ_CP063421:c226429-226112 [Vibrio cholerae]
ATGCAAAATAAACAATATAAGCTCAGCCAATTAGCCCAAGAGCACTTACTCAAGATTAAACACTACACCATTGAAAATTT
CGCTGAAGCGCAGTGGCAAAAATATAAGTCGACCTTGCTTTCAGGTTTCCAAACTCTAGCGGATAACCCAGGACTAGGAA
AAAGCTGTGAAGATATTTACCAAAATGGTTTTTACTTTCCAGTGGGGAAACACATGGCTTATTACACCAAAGAAGCAGAC
TGCATTCTCATTGTCGCGGTATTAGGGCAATCACAGCTGCCCCAAAAGCATCTCAAACAGTCACGCTTTGTTTCTTAG

Antitoxin

Protein

• Length: 200 a.a.
• Molecular weight: 22343.47 Da
• Isoelectric Point: 5.1445

>AT148070 WP_001251224.1 NZ_CP047815:c1882284-1881685 [Staphylococcus aureus]
MAMNFKVFDNSQLVAEYAADIIRKQFNNNPTTIAGFHLDTDQAPVLDELKKNVEKHAVDFSQINILDYDDKKSYFEALGV
PAGQVYPIAYEKDAIELIADKIKTKENKGKLTLQVVSIDEQGKLNVSIRQGLMEAREIFLVVTGANKRDVVEKLYQENGK
TSFEPADLKAHRMVNVILDKEAAAGLPEDVKAYFTSRFA

Nucleotide

• Length: 600 bp

>AT148070 NZ_CP063421:c226690-226448 [Vibrio cholerae]
ATGCACACACTAACAGCAAATGATGCTAAACGAAATTTTGGTGAACTTCTTCTAAGCGCACAGCGCGAACCTGTCATCAT
CAGCAAAAACAGTAAGAATACTGTCGTTGTCATGTCTATTAAGGACTTTGAAGAACTTGAAGCCATGAAACTCGATTATC
TCAAACACTGCTTTGAGTCTGCTCAGAAAGACTTAGATAGTGGCAAAACCGTTGATGGTGCCACTTTTCTAAACACCCTT
TGA

Structures

Toxin

• AlphaFold DB: X5E2E6

Antitoxin

• AlphaFold DB: W8U4V4