Detailed information of TA system TA148068

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2655256..2655785
• Replicon: chromosome
• Accession: NZ_CP047813
• Organism: Staphylococcus aureus strain UP_1500

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: E3T09_RS13075
• Protein ID: WP_000621175.1
• Coordinates: 2655423..2655785 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: E3T09_RS13070
• Protein ID: WP_000948331.1
• Coordinates: 2655256..2655426 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3T09_RS13040	2650292..2650852	+	561	WP_001092409.1	K(+)-transporting ATPase subunit C	-
E3T09_RS13045	2651061..2651540	+	480	WP_001287078.1	hypothetical protein	-
E3T09_RS13050	2651533..2653116	+	1584	WP_043044023.1	PH domain-containing protein	-
E3T09_RS13055	2653103..2653594	+	492	WP_072353916.1	PH domain-containing protein	-
E3T09_RS13060	2653598..2653957	+	360	WP_000581197.1	holo-ACP synthase	-
E3T09_RS13065	2654023..2655171	+	1149	WP_001281145.1	alanine racemase	-
E3T09_RS13070	2655256..2655426	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
E3T09_RS13075	2655423..2655785	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
E3T09_RS13080	2656134..2657135	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
E3T09_RS13085	2657254..2657580	+	327	WP_001052491.1	anti-sigma factor antagonist	-
E3T09_RS13090	2657582..2658061	+	480	WP_001190830.1	anti-sigma B factor RsbW	-
E3T09_RS13095	2658036..2658806	+	771	WP_001041107.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T148068 WP_000621175.1 NZ_CP047813:2655423-2655785 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T148068 NZ_CP063421:200928-201329 [Vibrio cholerae]
ATGGATATCATCTGTTTTCCTTTTGAACGAGTGATCGAGATTAATGCTTTCATCCTAAAAACAGAACCAGGAATGAAAGG
AGCCGTTGATATTCCCAAGCTTCAGGGGGCATTAGGTCGAATCGATAATGCCATCGTTTATGAAGGACTGGATGATGTTT
TTGAAATCGCTGCTAAATACACTGCTTGTATAGCGGTTTCACATGCGCTACCTGATGCGAACAAACGCACAGGTTTGGCT
GTGGCACTTGAATATTTATCACTTAACGATTTTGAGCTTACGCAGGAAAACGATTTACTTGCGGATGCAGTTCGAGATCT
TGTTATCGGCATTATCAACGAAACAGATTTTGCCGACATTCTCTACGCACAATACGCAAAAGAACAAAATTCAGTTCTCT
AA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT148068 WP_000948331.1 NZ_CP047813:2655256-2655426 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT148068 NZ_CP063421:200758-200928 [Vibrio cholerae]
ATGAATCGAAAAGTTGAAGCTTACGGCGTTGATGCTGTTGAAAGACCGAAAATTAAGGCTAGTAAAAAGCTTGATTTGAC
TGGTGATGCTGGTCGTCAAATTGTGAAGTCTGAAACTAAACTTGCTTTGCGTACTCATCAGAAGACATTCACTAAATTGG
CTGATATGTAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1