Detailed information of TA system TA148051

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2682316..2682845
• Replicon: chromosome
• Accession: NZ_CP047811
• Organism: Staphylococcus aureus strain UP_1522

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: E3T10_RS13255
• Protein ID: WP_000621175.1
• Coordinates: 2682483..2682845 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: E3T10_RS13250
• Protein ID: WP_000948331.1
• Coordinates: 2682316..2682486 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3T10_RS13220	2677353..2677913	+	561	WP_001092406.1	K(+)-transporting ATPase subunit C	-
E3T10_RS13225	2678121..2678600	+	480	WP_001287077.1	hypothetical protein	-
E3T10_RS13230	2678593..2680176	+	1584	WP_001294637.1	PH domain-containing protein	-
E3T10_RS13235	2680163..2680654	+	492	WP_001205907.1	PH domain-containing protein	-
E3T10_RS13240	2680658..2681017	+	360	WP_000581199.1	holo-ACP synthase	-
E3T10_RS13245	2681083..2682231	+	1149	WP_001281140.1	alanine racemase	-
E3T10_RS13250	2682316..2682486	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
E3T10_RS13255	2682483..2682845	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
E3T10_RS13260	2683194..2684195	+	1002	WP_042744356.1	PP2C family protein-serine/threonine phosphatase	-
E3T10_RS13265	2684314..2684640	+	327	WP_001052491.1	anti-sigma factor antagonist	-
E3T10_RS13270	2684642..2685121	+	480	WP_001190825.1	anti-sigma B factor RsbW	-
E3T10_RS13275	2685096..2685866	+	771	WP_001041107.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T148051 WP_000621175.1 NZ_CP047811:2682483-2682845 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T148051 NZ_CP063403:1791315-1791647 [Klebsiella variicola]
ATGGACAGAGGGGAAATCTGGCTTGTTTCACTGGATCCGATTGCTGGCCACGAGCAAAGTGGAAAACGTCCGGTCCTCAT
TGTATCGAAGGCATCGTTTAACAAGCTGACCCGGCTACCGGTCGTGGTTCCCGTCACCAGCGGCGGAAACTTTGCACGTA
CAGCAGGCTTCACGGTTTCACTGGAAGAGGCGGGGACCAAAACAACAGGCGTTATTCGCTGCGACCAGCCCAGAACTATC
GATATGGCGGCCCGGAACGGCAAGCGTCTGGAACGCATACCTGACGCCGTTGTCAATGAAGTGCTTGCCAGGCTGGATGC
GATTCTCAGTTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT148051 WP_000948331.1 NZ_CP047811:2682316-2682486 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT148051 NZ_CP063403:1791057-1791314 [Klebsiella variicola]
ATGTACAAAACTCGCCTGAAAAAGGTTGGCGGATCCATCATGCTGGCGGTTCCTCCCGCCGTGCTGAAAGCGCTGGAGCT
GTCGACAGACAGCGAAGTGGGTATGACTATAGATAATGGCTGCCTGATTATTGAACCGCAGAAACGGCCCCATTATTCGC
TTGAGGAACTGTTGGCGCAGTGCGATCCGCACGCCGAAATGAGCGAAGAGGATCGGGAATGGATTGATGCGCCTGCGGTG
GGCAAGGAGATTCTGTAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1