Detailed information of TA system TA148032

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1251639..1252168
• Replicon: chromosome
• Accession: NZ_CP047809
• Organism: Staphylococcus aureus strain UP_1591

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: E3T14_RS06260
• Protein ID: WP_000621175.1
• Coordinates: 1251639..1252001 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: E3T14_RS06265
• Protein ID: WP_000948331.1
• Coordinates: 1251998..1252168 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3T14_RS06240	1248617..1249387	-	771	WP_001041103.1	RNA polymerase sigma factor SigB	-
E3T14_RS06245	1249362..1249841	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
E3T14_RS06250	1249843..1250169	-	327	WP_001052491.1	anti-sigma factor antagonist	-
E3T14_RS06255	1250288..1251289	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
E3T14_RS06260	1251639..1252001	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
E3T14_RS06265	1251998..1252168	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
E3T14_RS06270	1252253..1253401	-	1149	WP_161575702.1	alanine racemase	-
E3T14_RS06275	1253467..1253826	-	360	WP_000581200.1	holo-ACP synthase	-
E3T14_RS06280	1253830..1254321	-	492	WP_001205910.1	PH domain-containing protein	-
E3T14_RS06285	1254308..1255891	-	1584	WP_001294626.1	PH domain-containing protein	-
E3T14_RS06290	1255884..1256363	-	480	WP_001287088.1	hypothetical protein	-
E3T14_RS06295	1256572..1257132	-	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T148032 WP_000621175.1 NZ_CP047809:c1252001-1251639 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T148032 NZ_CP063396:c5603401-5603123 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT148032 WP_000948331.1 NZ_CP047809:c1252168-1251998 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT148032 NZ_CP063396:c5603111-5602806 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTTGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1