Detailed information of TA system TA147961

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): omega-epsilon-zeta/zeta-epsilon
• Location: 1699619..1700756
• Replicon: chromosome
• Accession: NZ_CP047803
• Organism: Staphylococcus aureus strain UP_1097

Toxin (Protein)

• Gene name: zeta
• Uniprot ID: -
• Locus tag: E3S62_RS08545
• Protein ID: WP_075100294.1
• Coordinates: 1699619..1700482 (-)
• Length: 288 a.a.

Antitoxin (Protein)

• Gene name: epsilon
• Uniprot ID: Q9AL01
• Locus tag: E3S62_RS08550
• Protein ID: WP_002331065.1
• Coordinates: 1700484..1700756 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3S62_RS08515	1694642..1695040	-	399	WP_000415662.1	DUF4064 domain-containing protein	-
E3S62_RS08520	1695189..1695563	-	375	WP_000822169.1	DUF4467 domain-containing protein	-
E3S62_RS08525	1696184..1696672	-	489	WP_000142089.1	TIGR01741 family protein	-
E3S62_RS08530	1696683..1697183	-	501	WP_000142105.1	TIGR01741 family protein	-
E3S62_RS08535	1697249..1697923	+	675	WP_001105991.1	IS6-like element IS257 family transposase	-
E3S62_RS08540	1698860..1699177	-	318	WP_049287895.1	hypothetical protein	-
E3S62_RS08545	1699619..1700482	-	864	WP_075100294.1	zeta toxin family protein	Toxin
E3S62_RS08550	1700484..1700756	-	273	WP_002331065.1	antitoxin	Antitoxin
E3S62_RS08555	1700773..1700982	-	210	WP_000527318.1	peptide-binding protein	-
E3S62_RS08560	1701081..1701977	-	897	WP_002334978.1	ParA family protein	-
E3S62_RS08570	1702240..1702812	-	573	WP_001079938.1	HTH domain-containing protein	-
E3S62_RS08580	1703088..1703882	-	795	WP_001096887.1	aminoglycoside O-phosphotransferase APH(3')-IIIa	-
E3S62_RS08585	1703975..1704505	-	531	WP_000627289.1	streptothricin N-acetyltransferase Sat4	-
E3S62_RS08590	1704502..1705410	-	909	WP_160199765.1	aminoglycoside 6-adenylyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1697249..1697923	674

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (28-214)

Antitoxin

• Bacterial epsilon antitoxin (2-90)

Sequences

Toxin

Protein

• Length: 288 a.a.
• Molecular weight: 32611.17 Da
• Isoelectric Point: 6.6627

>T147961 WP_075100294.1 NZ_CP047803:c1700482-1699619 [Staphylococcus aureus]
MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGSGKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELV
KLYEKDVVKHVTPYSNRMTEALISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSKGYETKIYVMAVPKIESYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLERELNRKVSGKEIQ
PTLERIEQKMVQNQHQETPEFKAIQQKMESLQLPTPPIPKTPKLPGL

Nucleotide

• Length: 864 bp

>T147961 NZ_CP063385:c143118-142837 [Pseudomonas aeruginosa]
ATGAGCCTGAAGTGGACCCGCAAGGCGGCCGCCGACCTGGACGCCATCTACGACCATTACGTCGTGCTGATCGGCCCGGA
AAAAGCCCTGAAAGCCGTCCAGGACATCGTCGAGCAGGTGAAACCGCTGCAGCAGGTAGCCAACCAGGGGGCAGGGCGGC
CCAGCGAGGTGCCAGGCGTACGCACCCTGACCCTGGAGCGCTGGCCGTTCAGCGCCCCTTTTCGGGTAAAAGGCAAGGAA
ATCCAGATTTTGCGCATCGACAGGGTCGAAATTACCCCCTAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10733.17 Da
• Isoelectric Point: 4.4865

>AT147961 WP_002331065.1 NZ_CP047803:c1700756-1700484 [Staphylococcus aureus]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEVNLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYS
KQILNKEKVA

Nucleotide

• Length: 273 bp

>AT147961 NZ_CP063385:c143342-143115 [Pseudomonas aeruginosa]
ATGAGCACCGTAGTCTCGTTCCGCGCCGATGACGCCCTGGTCGCGGCCCTCGACGAACTGGCCCGCGCCACCCACCGCGA
CCGCCCGTATCACCTGCGGCAGGCGCTCGCGCAGTACCTGGAAAGGCAGCAGTGGCAGGTCGCTGCCATCGATGAAGGCT
TGGCCGATGCCAATGCCGGTCGCCTGCTGGAACACACCGAGATCGAGAAGCGCTGGGGGCTGCAATGA

Structures

Antitoxin

• AlphaFold DB: A0A828ZL55