Detailed information of TA system TA147946

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1805985..1806514
• Replicon: chromosome
• Accession: NZ_CP047802
• Organism: Staphylococcus aureus strain UP_1442

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: E3S67_RS09205
• Protein ID: WP_000621175.1
• Coordinates: 1805985..1806347 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: E3S67_RS09210
• Protein ID: WP_000948331.1
• Coordinates: 1806344..1806514 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3S67_RS09185	1802964..1803734	-	771	WP_001041111.1	RNA polymerase sigma factor SigB	-
E3S67_RS09190	1803709..1804188	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
E3S67_RS09195	1804190..1804516	-	327	WP_001052491.1	anti-sigma factor antagonist	-
E3S67_RS09200	1804635..1805636	-	1002	WP_000390827.1	PP2C family protein-serine/threonine phosphatase	-
E3S67_RS09205	1805985..1806347	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
E3S67_RS09210	1806344..1806514	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
E3S67_RS09215	1806599..1807747	-	1149	WP_001281154.1	alanine racemase	-
E3S67_RS09220	1807813..1808172	-	360	WP_000581197.1	holo-ACP synthase	-
E3S67_RS09225	1808176..1808667	-	492	WP_001205912.1	PH domain-containing protein	-
E3S67_RS09230	1808654..1810237	-	1584	WP_001294626.1	PH domain-containing protein	-
E3S67_RS09235	1810230..1810709	-	480	WP_001287087.1	hypothetical protein	-
E3S67_RS09240	1810917..1811477	-	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T147946 WP_000621175.1 NZ_CP047802:c1806347-1805985 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T147946 NZ_CP063380:c93274-92978 [Pectobacterium versatile]
ATGACATCATGGAATCGGTCATGCAGGGGAAAAATAGCCAGCCTACGAAAGAAGCAGCGGGAAACGCTGAGCCACATTTT
CAAAACACCAATACTTTCAGGTGTTAAATGGTCGAGTATTGAGTCACTCGTTACTGCGTTAGGCGGTGAAATTAAGGAAG
GTAGCGGGTCACGAGTGCGATTTTTATTGAACGGGAGTATCGCCCGATTCCATCGTCCTCATCCGTCACCGGATACTGAT
AAAGGTGCCCTGGTTAGCCTGCGTGAATGGCTGGAAAGTATAGGAGTAAAACCATGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT147946 WP_000948331.1 NZ_CP047802:c1806514-1806344 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT147946 NZ_CP063380:c92981-92619 [Pectobacterium versatile]
ATGACGAAAGCACTCAACACACCAAATACGATGGTTGTTTCTGGACAACCTGCCATTATCAGTTACGTACCGGAAATTGG
TATGTTTCGTGGTAAGTTTATTGGCTTATCTGGCTACTGTGATTTTGTCGCCGACAGTATTAATGGTTTGAGAAGCGAGG
GTGAAATTTCGCTACGCGAATATCTGGAAGACTGTCAGGAAAATGGCATCGAACCTTACGAGCGTGAAGAGAAGGTGAAA
ACATTCACCCTCCGTTACCCTGAATCATTCGGGGAACGACTGACCATTGCGGCCGCTGAACGTCACATTTCAGTGAATAC
GTTTATTGTTGAAACGCTTAGCGAACACCTGAAACAGGCTTGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1