Detailed information of TA system TA147914

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsdAT/-
• Location: 2723295..2723809
• Replicon: chromosome
• Accession: NZ_CP047800
• Organism: Staphylococcus aureus strain UP_296

Toxin (Protein)

• Gene name: TsdT
• Uniprot ID: T1YA99
• Locus tag: E3S72_RS13580
• Protein ID: WP_000581792.1
• Coordinates: 2723600..2723809 (+)
• Length: 70 a.a.

Antitoxin (Protein)

• Gene name: TsdA
• Uniprot ID: W8UW13
• Locus tag: E3S72_RS13575
• Protein ID: WP_000138932.1
• Coordinates: 2723295..2723588 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3S72_RS13540	2718458..2719099	+	642	WP_000483153.1	thiamine phosphate synthase	-
E3S72_RS13545	2719185..2720057	+	873	WP_000725802.1	membrane protein insertase YidC	-
E3S72_RS13565	2720970..2721617	-	648	WP_160198905.1	HD domain-containing protein	-
E3S72_RS13570	2721645..2723129	-	1485	WP_000571549.1	cardiolipin synthase	-
E3S72_RS13575	2723295..2723588	+	294	WP_000138932.1	copper-sensing transcriptional repressor CsoR	Antitoxin
E3S72_RS13580	2723600..2723809	+	210	WP_000581792.1	heavy-metal-associated domain-containing protein	Toxin
E3S72_RS13585	2723937..2724074	+	138	WP_000828354.1	hypothetical protein	-
E3S72_RS13590	2724338..2725540	-	1203	WP_001109939.1	rod shape-determining protein RodA	-
E3S72_RS13595	2725858..2726928	+	1071	WP_000159631.1	D-alanine--D-alanine ligase	-
E3S72_RS13600	2726943..2728301	+	1359	WP_000611464.1	UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Heavy metal-associated domain, HMA (23-66)

Antitoxin

• Metal-sensitive transcriptional repressor (14-92)

Sequences

Toxin

Protein

• Length: 70 a.a.
• Molecular weight: 7843.77 Da
• Isoelectric Point: 4.1008

>T147914 WP_000581792.1 NZ_CP047800:2723600-2723809 [Staphylococcus aureus]
MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVTVSYETPANLNSIEKEIYDEGYKIVF

Nucleotide

• Length: 210 bp

>T147914 NZ_CP063362:6228658-6228957 [Xanthobacter sp. YN2]
GTGCGAAGGCTTGAGTGGCGCGAGGACGCACGCGCCGATCTGCTGGCGATTGTCGATTTCATTTCCGACGACAACCCCGA
TGCCTCCCAACGGCTTAAGGATGACATAGAGGCGAAGACGTCAAAGTTGCCTGAGCATCCGAGGCTCTACCGCCCCGGCC
GCGTCGACGGCACGCGGGAAATGGTCGTGCGATCAAACTACCTGATCGTGTACGCCGAGAATGCCCACGCCATCTCCATT
CTGCGCGTCCTGCATGCGGCCCAGCAATGGCCGCCAGTGCCGGAGGACGAGGAGATCTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11197.82 Da
• Isoelectric Point: 6.2243

>AT147914 WP_000138932.1 NZ_CP047800:2723295-2723588 [Staphylococcus aureus]
MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDVLTQIRATRSALNSVAIKLLEQHMKSCIMNKVNQGAQ
EEAMEELLVTFQKLIKD

Nucleotide

• Length: 294 bp

>AT147914 NZ_CP063362:6228372-6228671 [Xanthobacter sp. YN2]
ATGGCCGCTCAAACCTCGATGCTGCACATCCGCGTCGACGACAAGCTGAAGGCCGACGCGACCGAGAAGCTGGCCAGCTT
CGGCCTGACGGTTTCCGATGCCGTCCGGATTCTGCTCACGCGCGTCGTAAAAGAGGGCGCGTTGCCGGCGGGCTTGACCA
CGGACCCGGAAGCGCATGACGCGTGGTTTCGGGCCAAGGTGCGAGAAGCCTTGGACGACACCCGGCCATCGGTGCCGCAC
CGGCAGGTGATGGATGAGACACAGGCGCTGATCGATAGGAAGCGCCGTGCGAAGGCTTGA

Structures

Toxin

• PDB: 6FF1

Antitoxin

• AlphaFold DB: W8UW13