Detailed information of TA system TA147878

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2677847..2678376
• Replicon: chromosome
• Accession: NZ_CP047798
• Organism: Staphylococcus aureus strain UP_378

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: E3S75_RS13185
• Protein ID: WP_000621175.1
• Coordinates: 2678014..2678376 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: E3S75_RS13180
• Protein ID: WP_000948331.1
• Coordinates: 2677847..2678017 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3S75_RS13150	2672884..2673444	+	561	WP_001092406.1	K(+)-transporting ATPase subunit C	-
E3S75_RS13155	2673652..2674131	+	480	WP_001287077.1	hypothetical protein	-
E3S75_RS13160	2674124..2675707	+	1584	WP_001294637.1	PH domain-containing protein	-
E3S75_RS13165	2675694..2676185	+	492	WP_001205907.1	PH domain-containing protein	-
E3S75_RS13170	2676189..2676548	+	360	WP_000581199.1	holo-ACP synthase	-
E3S75_RS13175	2676614..2677762	+	1149	WP_001281140.1	alanine racemase	-
E3S75_RS13180	2677847..2678017	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
E3S75_RS13185	2678014..2678376	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
E3S75_RS13190	2678725..2679726	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
E3S75_RS13195	2679845..2680171	+	327	WP_001052491.1	anti-sigma factor antagonist	-
E3S75_RS13200	2680173..2680652	+	480	WP_001190825.1	anti-sigma B factor RsbW	-
E3S75_RS13205	2680627..2681397	+	771	WP_001041107.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T147878 WP_000621175.1 NZ_CP047798:2678014-2678376 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T147878 NZ_CP063349:533759-534223 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT147878 WP_000948331.1 NZ_CP047798:2677847-2678017 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT147878 NZ_CP063349:533424-533759 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTCTTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1