Detailed information of TA system TA147858

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /HTH_19(antitoxin)
• Location: 1237541..1238329
• Replicon: chromosome
• Accession: NZ_CP047797
• Organism: Staphylococcus aureus strain UP_426

Toxin (Protein)

• Gene name: -
• Uniprot ID: A0A2C9TYR5
• Locus tag: E3S77_RS06190
• Protein ID: WP_001573840.1
• Coordinates: 1237868..1238329 (+)
• Length: 154 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A0E7YIA0
• Locus tag: E3S77_RS06185
• Protein ID: WP_000333630.1
• Coordinates: 1237541..1237855 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3S77_RS06130	1232781..1233560	-	780	WP_107399871.1	ATP-binding protein	-
E3S77_RS06135	1233553..1233774	-	222	WP_001077280.1	DUF2483 family protein	-
E3S77_RS06140	1233784..1234044	-	261	WP_043951459.1	DUF1108 family protein	-
E3S77_RS06145	1234138..1234299	-	162	WP_000048124.1	DUF1270 family protein	-
E3S77_RS06150	1234292..1234513	-	222	WP_000977381.1	hypothetical protein	-
E3S77_RS06155	1234527..1234976	-	450	WP_000993183.1	hypothetical protein	-
E3S77_RS06160	1234992..1235744	-	753	WP_043951460.1	phage antirepressor KilAC domain-containing protein	-
E3S77_RS06165	1235746..1235931	-	186	WP_000933365.1	helix-turn-helix transcriptional regulator	-
E3S77_RS06170	1236377..1236859	+	483	WP_000394410.1	hypothetical protein	-
E3S77_RS06175	1236960..1237139	-	180	WP_000438352.1	hypothetical protein	-
E3S77_RS06180	1237153..1237389	-	237	WP_001121116.1	helix-turn-helix domain-containing protein	-
E3S77_RS06185	1237541..1237855	+	315	WP_000333630.1	helix-turn-helix domain-containing protein	Antitoxin
E3S77_RS06190	1237868..1238329	+	462	WP_001573840.1	phage protein	Toxin
E3S77_RS06195	1238513..1239217	+	705	WP_160195323.1	type II toxin-antitoxin system PemK/MazF family toxin	-
E3S77_RS06200	1239414..1240463	+	1050	WP_001145730.1	site-specific integrase	-
E3S77_RS06205	1240531..1241928	-	1398	WP_001074405.1	Fe-S cluster assembly protein SufB	-
E3S77_RS06210	1242080..1242544	-	465	WP_001010508.1	SUF system NifU family Fe-S cluster assembly protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	eta	1191552..1241928	50376

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (4-62)

Sequences

Toxin

Protein

• Length: 154 a.a.
• Molecular weight: 18060.43 Da
• Isoelectric Point: 4.6915

>T147858 WP_001573840.1 NZ_CP047797:1237868-1238329 [Staphylococcus aureus]
MGLYEETLIQHDYIEVREADVLPDNLDGVWLGDLILIKRGLSDREKAGILFEELAHNKLTYGDIADYSKFNNRKFENYAR
RHGFISAVPLREIVEAYNYGVRNLYELSEYLQLSEEYILEAIEQYKKIYGIGTHYGEYSITFEPLRVFKYKEI

Nucleotide

• Length: 462 bp

>T147858 NZ_CP063341:1839163-1839417 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 12063.56 Da
• Isoelectric Point: 6.4736

>AT147858 WP_000333630.1 NZ_CP047797:1237541-1237855 [Staphylococcus aureus]
MCFSKRMKQSREKQGMTLAELGRKIGKTEATVQRYESGNIKNLKNDTIESIATALNVNPAYLMGWVEENDDEVQHRAAHL
EGELTDDEWQRVLDYADYIRSKRK

Nucleotide

• Length: 315 bp

>AT147858 NZ_CP063341:1838915-1839166 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATATAACTCCCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Structures

Toxin

• AlphaFold DB: A0A2C9TYR5

Antitoxin

• AlphaFold DB: A0A0E7YIA0