Detailed information of TA system TA147847

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /HTH_19(antitoxin)
• Location: 2374076..2374864
• Replicon: chromosome
• Accession: NZ_CP047796
• Organism: Staphylococcus aureus strain UP_462

Toxin (Protein)

• Gene name: -
• Uniprot ID: A0A2I7Y5B3
• Locus tag: E3S78_RS11405
• Protein ID: WP_000525004.1
• Coordinates: 2374076..2374537 (-)
• Length: 154 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: E3S78_RS11410
• Protein ID: WP_160176214.1
• Coordinates: 2374550..2374864 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3S78_RS11380	2369812..2370342	+	531	WP_000184383.1	acyl-CoA thioesterase	-
E3S78_RS11385	2370602..2371747	-	1146	WP_042907213.1	radical SAM/CxCxxxxC motif protein YfkAB	-
E3S78_RS11390	2371792..2372838	-	1047	WP_001145732.1	site-specific integrase	-
E3S78_RS11395	2372908..2373378	-	471	WP_000183249.1	hypothetical protein	-
E3S78_RS11400	2373371..2373892	-	522	WP_001102446.1	hypothetical protein	-
E3S78_RS11405	2374076..2374537	-	462	WP_000525004.1	hypothetical protein	Toxin
E3S78_RS11410	2374550..2374864	-	315	WP_160176214.1	helix-turn-helix domain-containing protein	Antitoxin
E3S78_RS11415	2375016..2375252	+	237	WP_001121116.1	helix-turn-helix domain-containing protein	-
E3S78_RS11420	2375538..2376020	-	483	WP_000394410.1	hypothetical protein	-
E3S78_RS11425	2376465..2376650	+	186	WP_000933365.1	helix-turn-helix transcriptional regulator	-
E3S78_RS11430	2376652..2377392	+	741	WP_031783118.1	phage antirepressor KilAC domain-containing protein	-
E3S78_RS11435	2377568..2377798	-	231	WP_000395455.1	hypothetical protein	-
E3S78_RS11440	2377869..2378090	+	222	WP_000594789.1	hypothetical protein	-
E3S78_RS11445	2378083..2378244	+	162	WP_000066011.1	DUF1270 domain-containing protein	-
E3S78_RS11450	2378341..2378601	+	261	WP_000291090.1	DUF1108 family protein	-
E3S78_RS11455	2378611..2378832	+	222	WP_001077280.1	DUF2483 family protein	-
E3S78_RS11460	2378825..2379448	+	624	WP_000139720.1	DUF1071 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2371792..2413872	42080

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (4-62)

Sequences

Toxin

Protein

• Length: 154 a.a.
• Molecular weight: 18074.46 Da
• Isoelectric Point: 4.6915

>T147847 WP_000525004.1 NZ_CP047796:c2374537-2374076 [Staphylococcus aureus]
MGLYEETLIQHDYIEIREADVLPDNLDGVWLGDLILIKRGLSDREKAGILFEELAHNKLTYGDIADYSKFNNRKFENYAR
RHGFISAVPLREIVEAYNYGVRNLYELSEYLQLSEEYILEAIEQYKKIYGIGTHYGEYSITFEPLRVFKYKEI

Nucleotide

• Length: 462 bp

>T147847 NZ_CP063337:c96327-96169 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 12109.65 Da
• Isoelectric Point: 6.4674

>AT147847 WP_160176214.1 NZ_CP047796:c2374864-2374550 [Staphylococcus aureus]
MCFSKRMKQSREKQCMTLAELGRKIGKTEATVQRYESGNIKNLKNDTIESIATALNVNPAYLMGWVEENDDEVQHRAAHL
EGELTDDEWQRVLDYADYIRSKRK

Nucleotide

• Length: 315 bp

>AT147847 NZ_CP063337:c96595-96371 [Escherichia coli]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: A0A2I7Y5B3