147846

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2247626..2248155 Replicon chromosome
Accession NZ_CP047796
Organism Staphylococcus aureus strain UP_462

Toxin (Protein)


Gene name mazF Uniprot ID Q6GF05
Locus tag E3S78_RS10770 Protein ID WP_000621176.1
Coordinates 2247793..2248155 (+) Length 121 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID T1YCG8
Locus tag E3S78_RS10765 Protein ID WP_000948331.1
Coordinates 2247626..2247796 (+) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E3S78_RS10735 2242662..2243222 + 561 WP_001092414.1 K(+)-transporting ATPase subunit C -
E3S78_RS10740 2243431..2243910 + 480 WP_001287082.1 hypothetical protein -
E3S78_RS10745 2243903..2245480 + 1578 WP_001294646.1 PH domain-containing protein -
E3S78_RS10750 2245473..2245964 + 492 WP_001286801.1 PH domain-containing protein -
E3S78_RS10755 2245968..2246327 + 360 WP_000581197.1 holo-ACP synthase -
E3S78_RS10760 2246393..2247541 + 1149 WP_001281140.1 alanine racemase -
E3S78_RS10765 2247626..2247796 + 171 WP_000948331.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
E3S78_RS10770 2247793..2248155 + 363 WP_000621176.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
E3S78_RS10775 2248504..2249505 + 1002 WP_000390829.1 PP2C family protein-serine/threonine phosphatase -
E3S78_RS10780 2249624..2249950 + 327 WP_001052491.1 anti-sigma factor antagonist -
E3S78_RS10785 2249952..2250431 + 480 WP_001190825.1 anti-sigma B factor RsbW -
E3S78_RS10790 2250406..2251176 + 771 WP_001041107.1 RNA polymerase sigma factor SigB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-110)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 121 a.a.        Molecular weight: 13469.74 Da        Isoelectric Point: 10.1654

>T147846 WP_000621176.1 NZ_CP047796:2247793-2248155 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVVHQKN

Download         Length: 363 bp

>T147846 NZ_CP063337:c58981-58694 [Escherichia coli]
ATGACTTATACGGTAAAATTCAGGGATGATGCGCTCAAGGAATGGCTGAAACTGGATAAGACCATTCAACAACAGTTTGT
GAAAAAACTAAAAAAATGCAGCGAAAATCCTCATATACCGTCAGCAAAACTAAGAGGGCTAAAGGATTGCTACAAAATTA
AACTACGTGCATCGGGATTTCGTCTGGTTTATCAGGTGATTGACGATATGTTAATTATTGCGGTTGTTGCGGTAGGAAAA
CGAGAACGTAGTAACGTTTATAATCTCGCCAGTGAGAGAATGAGATAG

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6251.78 Da        Isoelectric Point: 3.8305

>AT147846 WP_000948331.1 NZ_CP047796:2247626-2247796 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Download         Length: 171 bp

>AT147846 NZ_CP063337:c59229-58978 [Escherichia coli]
ATGCCTAACATCATTCTGTCTGACACCAGTGCAAGTGTCAGTGAACTGAAAAAAAATCCTATGGCTACAGTCAGTGCCGG
AGATGGTTACCCTGTCGCCATTCTTAACCGGAATCAGCCGGCATTCTACTGTGTGCCTGCTGAACTGTATGAAAGGATGC
TTGATGCTCTGGATGACCAGGAACTGGTGAAACTGGTTACTGAACGCAGCAATCAGCCACTGCATGATGTGGACCTAGAC
AGTTATTTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A168PYX0

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0VRZ1

References