Detailed information of TA system TA147829

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2035315..2035844
• Replicon: chromosome
• Accession: NZ_CP047795
• Organism: Staphylococcus aureus strain UP_490

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: E3S79_RS10015
• Protein ID: WP_000621175.1
• Coordinates: 2035315..2035677 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: E3S79_RS10020
• Protein ID: WP_000948331.1
• Coordinates: 2035674..2035844 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3S79_RS09995	2032294..2033064	-	771	WP_001041107.1	RNA polymerase sigma factor SigB	-
E3S79_RS10000	2033039..2033518	-	480	WP_001190825.1	anti-sigma B factor RsbW	-
E3S79_RS10005	2033520..2033846	-	327	WP_001052491.1	anti-sigma factor antagonist	-
E3S79_RS10010	2033965..2034966	-	1002	WP_042744356.1	PP2C family protein-serine/threonine phosphatase	-
E3S79_RS10015	2035315..2035677	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
E3S79_RS10020	2035674..2035844	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
E3S79_RS10025	2035929..2037077	-	1149	WP_001281140.1	alanine racemase	-
E3S79_RS10030	2037143..2037502	-	360	WP_000581199.1	holo-ACP synthase	-
E3S79_RS10035	2037506..2037997	-	492	WP_160192119.1	PH domain-containing protein	-
E3S79_RS10040	2037984..2039567	-	1584	WP_001294637.1	PH domain-containing protein	-
E3S79_RS10045	2039560..2040039	-	480	WP_001287077.1	hypothetical protein	-
E3S79_RS10050	2040247..2040807	-	561	WP_001092406.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T147829 WP_000621175.1 NZ_CP047795:c2035677-2035315 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T147829 NZ_CP063332:c1900131-1899796 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT147829 WP_000948331.1 NZ_CP047795:c2035844-2035674 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT147829 NZ_CP063332:c1900379-1900131 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1