Detailed information of TA system TA147777

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1764962..1765491
• Replicon: chromosome
• Accession: NZ_CP047792
• Organism: Staphylococcus aureus strain UP_794

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: E3S88_RS08830
• Protein ID: WP_000621175.1
• Coordinates: 1764962..1765324 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: E3S88_RS08835
• Protein ID: WP_000948331.1
• Coordinates: 1765321..1765491 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3S88_RS08810	1761940..1762710	-	771	WP_001041107.1	RNA polymerase sigma factor SigB	-
E3S88_RS08815	1762685..1763164	-	480	WP_001190825.1	anti-sigma B factor RsbW	-
E3S88_RS08820	1763166..1763492	-	327	WP_001052491.1	anti-sigma factor antagonist	-
E3S88_RS08825	1763611..1764612	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
E3S88_RS08830	1764962..1765324	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
E3S88_RS08835	1765321..1765491	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
E3S88_RS08840	1765576..1766724	-	1149	WP_124779899.1	alanine racemase	-
E3S88_RS08845	1766790..1767149	-	360	WP_000581197.1	holo-ACP synthase	-
E3S88_RS08850	1767153..1767644	-	492	WP_124779897.1	PH domain-containing protein	-
E3S88_RS08855	1767631..1769214	-	1584	WP_124779895.1	PH domain-containing protein	-
E3S88_RS08860	1769207..1769686	-	480	WP_124779893.1	hypothetical protein	-
E3S88_RS08865	1769894..1770454	-	561	WP_124779891.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T147777 WP_000621175.1 NZ_CP047792:c1765324-1764962 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T147777 NZ_CP063242:c4306278-4305973 [Pectobacterium carotovorum]
ATGCCACAAGTGATCGTTTCAGAACGCGCGAGGCGTGATTTGCAGCGTCTACAGGATTTTCTAAAAAACAAAAATGCGCT
GGCCGCGAAAAAGGCCGCTGAAGTTTTGATTCGGGGCATTCGTCAGTTAGAAGCGCTGCCGAATATCGGGCGACCAGTGG
AACATCTTCCCCTTGAATATCAAGAGTTGGTGATCGAATTCGGCAGCAGCGGGTATGTTATGCTTTACCGTCACGATGAG
CTGACCGGCAGAGTGGTGATACTGACCGTTAAACATCAAAAAGAAGCTGGTTATCAAAACCCGTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT147777 WP_000948331.1 NZ_CP047792:c1765491-1765321 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT147777 NZ_CP063242:c4306556-4306266 [Pectobacterium carotovorum]
ATGGCTGACACCACACGTCAAACCACGACGCTGCGCATCGATCAGGACATTAAAGAACGACTGAAAACGCTGGCCGATGA
CCGCAACAGCTCAGCCCATGCGCTGATGCTGGAAGCGATTGCCGAATACGTTGAACGGGAAGAAAAACGCAGCCAGTATC
GTAAAGAGGCGTTGGCCGCGTGGGAAGACTATCAGGACACTGGCAAACATATCACCGCAGATGAGACCGTTACCTGGTTA
GAATCCTGGGGTTCTGGACATGAACAGGATGCGCCTGCATGCCACAAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1