Detailed information of TA system TA147750

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2672829..2673358
• Replicon: chromosome
• Accession: NZ_CP047790
• Organism: Staphylococcus aureus strain UP_830

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: E3S90_RS13125
• Protein ID: WP_000621175.1
• Coordinates: 2672996..2673358 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: E3S90_RS13120
• Protein ID: WP_000948331.1
• Coordinates: 2672829..2672999 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3S90_RS13090	2667865..2668425	+	561	WP_001092402.1	K(+)-transporting ATPase subunit C	-
E3S90_RS13095	2668634..2669113	+	480	WP_001287090.1	hypothetical protein	-
E3S90_RS13100	2669106..2670689	+	1584	WP_001294626.1	PH domain-containing protein	-
E3S90_RS13105	2670676..2671167	+	492	WP_153081068.1	PH domain-containing protein	-
E3S90_RS13110	2671171..2671530	+	360	WP_000581197.1	holo-ACP synthase	-
E3S90_RS13115	2671596..2672744	+	1149	WP_001281142.1	alanine racemase	-
E3S90_RS13120	2672829..2672999	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
E3S90_RS13125	2672996..2673358	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
E3S90_RS13130	2673708..2674709	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
E3S90_RS13135	2674828..2675154	+	327	WP_001052491.1	anti-sigma factor antagonist	-
E3S90_RS13140	2675156..2675635	+	480	WP_001190829.1	anti-sigma B factor RsbW	-
E3S90_RS13145	2675610..2676380	+	771	WP_001041102.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T147750 WP_000621175.1 NZ_CP047790:2672996-2673358 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T147750 NZ_CP063238:4281048-4281392 [Serratia marcescens]
ATGTGGCAAGTGGTGACCGTCGAACGGTTCGACGACTGGTTTTTAGCGCTGAACAACGCCCAGCAAACAAGCATCCTGGC
GGCGATCTTCAAATTGCAGACGTTCGGCCCGCAGTTGACGCGGCCGCATGCCGATACGCTGTATTTTTCCGACGCGGTCC
GGCAGTTGAAGGAGCTGCGCGTTCAACATCGCGGGCGCCCTTTCAGGGTGTTTTTCGCCTTTGACCTACAGCGGCAAGCG
GTATTGCTGTGCGGTGGCGATAAAACCGGCGACAAACGCTTTTACCAGCGCATGTTGCCCATCGCCGCCATGGAGTTTTC
ACATTATCTGGCAACCCGGAGGTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT147750 WP_000948331.1 NZ_CP047790:2672829-2672999 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT147750 NZ_CP063238:4281398-4281700 [Serratia marcescens]
ATGGCCAAACCTTTATCCCAACTGATCGATAAACTCGATCCCGCCGTCGTTGCCGCCGCCAGGCAAAAAGCGGATCAGGA
AATTTTCGAGCTGCGGCTGGCGATGCTGAGGGAAGAGCTGGCGGTCTCTCAGGTTGAATTGGCGAAGCGGCTCGGCATCA
GCCAACCCTCCGTCGCCAATTTGGAAAAGCGCGGCAGCGAAATAAAGCTGTCGTCGCTCAAACGCTATATCGAGGCCATG
GGCGGCACGCTGTCGCTGGATGTGCAATTGCCCAATGGCCAACACCGCCGCATGACGCTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1