Detailed information of TA system TA147733

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2724597..2725126
• Replicon: chromosome
• Accession: NZ_CP047789
• Organism: Staphylococcus aureus strain UP_967

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: E3S93_RS13595
• Protein ID: WP_000621175.1
• Coordinates: 2724764..2725126 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: E3S93_RS13590
• Protein ID: WP_000948331.1
• Coordinates: 2724597..2724767 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3S93_RS13560	2719634..2720194	+	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-
E3S93_RS13565	2720402..2720881	+	480	WP_001287088.1	hypothetical protein	-
E3S93_RS13570	2720874..2722457	+	1584	WP_001294626.1	PH domain-containing protein	-
E3S93_RS13575	2722444..2722935	+	492	WP_001205910.1	PH domain-containing protein	-
E3S93_RS13580	2722939..2723298	+	360	WP_000581200.1	holo-ACP synthase	-
E3S93_RS13585	2723364..2724512	+	1149	WP_001281145.1	alanine racemase	-
E3S93_RS13590	2724597..2724767	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
E3S93_RS13595	2724764..2725126	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
E3S93_RS13600	2725476..2726477	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
E3S93_RS13605	2726596..2726922	+	327	WP_001052491.1	anti-sigma factor antagonist	-
E3S93_RS13610	2726924..2727403	+	480	WP_001190829.1	anti-sigma B factor RsbW	-
E3S93_RS13615	2727378..2728148	+	771	WP_001041103.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T147733 WP_000621175.1 NZ_CP047789:2724764-2725126 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T147733 NZ_CP063229:5056516-5056866 [Serratia marcescens]
ATGGTGAAGCGCCCCGTTTTTCAGCGCGGAGATCTTATCCGGGTCAGCCTGAATCCCGTGGTTGGCCGTGAGCAGCAAGG
CGACTTTCGTCCGGCACTGGTGCTCTCTCCCCGCGAGTTTAATGCGCTGGGCATGGTGCTGGTTGCCCCTATTTCACAAG
GGGCTAATTTTGCCCGTACAGCGGGTTTCACCGTCAGCCTGAACGGGAGCGGCACCGCAACGCAGGGCGTCATCTTGATC
AACCAGGTTCGCATGATGGATCTGGCCGGGCGCAGCGCGCAATTCGTTGAACGTGTCCCTGGCGAAGTCATCGCCGACGC
GCTGGCGCGGCTTCAGGCAATCATCGACTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT147733 WP_000948331.1 NZ_CP047789:2724597-2724767 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT147733 NZ_CP063229:5056274-5056522 [Serratia marcescens]
ATGAAAGTCTTTATCAAAAAATGGGGCAACAGCGCGGGAATGCTGGTGCCCTCAGCGCTAATGAACGAGCTGGAACTACA
GGTAGGTCAGGAAATGGACATGCAGGTCATCGATGGAACGTTGGTCATTAAACCGGTTAAGAAACGCTATTCACTGCAGC
AATTGCTCGATGGCTGTGACGGTGAAGCGCCGGCCGCCGCAGACGACAACTGGGAACACATGCCGCCGGCGGGGAAAGAA
GTATGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1