Detailed information of TA system TA147684

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2696920..2697449
• Replicon: chromosome
• Accession: NZ_CP047786
• Organism: Staphylococcus aureus strain UP_1150

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: E3S98_RS13215
• Protein ID: WP_000621175.1
• Coordinates: 2697087..2697449 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: E3S98_RS13210
• Protein ID: WP_000948331.1
• Coordinates: 2696920..2697090 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3S98_RS13180	2691956..2692516	+	561	WP_001092409.1	K(+)-transporting ATPase subunit C	-
E3S98_RS13185	2692725..2693204	+	480	WP_001287078.1	hypothetical protein	-
E3S98_RS13190	2693197..2694780	+	1584	WP_045173493.1	PH domain-containing protein	-
E3S98_RS13195	2694767..2695258	+	492	WP_078103494.1	PH domain-containing protein	-
E3S98_RS13200	2695262..2695621	+	360	WP_000581200.1	holo-ACP synthase	-
E3S98_RS13205	2695687..2696835	+	1149	WP_001281145.1	alanine racemase	-
E3S98_RS13210	2696920..2697090	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
E3S98_RS13215	2697087..2697449	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
E3S98_RS13220	2697799..2698800	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
E3S98_RS13225	2698919..2699245	+	327	WP_001052491.1	anti-sigma factor antagonist	-
E3S98_RS13230	2699247..2699726	+	480	WP_001190829.1	anti-sigma B factor RsbW	-
E3S98_RS13235	2699701..2700471	+	771	WP_001041103.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T147684 WP_000621175.1 NZ_CP047786:2697087-2697449 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T147684 NZ_CP063214:2314351-2314728 [Escherichia coli]
ATGAACACATTACCCGACACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTCACGCTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTACGAACTGACCAGCCAGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGACCCGCGA
CAATTACAGAATGGTAAATAACATTACCCAGGGTAAGCATCCGGAGGCGCAACAATGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT147684 WP_000948331.1 NZ_CP047786:2696920-2697090 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT147684 NZ_CP063214:2313893-2314261 [Escherichia coli]
GTGTCAGACACACTGCCCGGGACAACGCATCCCGACGACAATCACGACCGCCTCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGAAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGATGCAGATGCATACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAACGTAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1