Detailed information of TA system TA147673

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tsbAT/-
• Location: 121494..122270
• Replicon: chromosome
• Accession: NZ_CP047786
• Organism: Staphylococcus aureus strain UP_1150

Toxin (Protein)

• Gene name: tsbT
• Uniprot ID: X5E2E6
• Locus tag: E3S98_RS00775
• Protein ID: WP_000031108.1
• Coordinates: 122118..122270 (+)
• Length: 51 a.a.

Antitoxin (Protein)

• Gene name: tsbA
• Uniprot ID: W8U4V4
• Locus tag: E3S98_RS00770
• Protein ID: WP_001251224.1
• Coordinates: 121494..122093 (+)
• Length: 200 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3S98_RS00750	117411..118868	+	1458	WP_000649908.1	ABC transporter permease subunit	-
E3S98_RS00755	118861..119583	+	723	WP_000590809.1	amino acid ABC transporter ATP-binding protein	-
E3S98_RS00760	119734..120861	+	1128	WP_201750720.1	tRNA epoxyqueuosine(34) reductase QueG	-
E3S98_RS00765	120866..121336	+	471	WP_000181394.1	tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL	-
E3S98_RS00770	121494..122093	+	600	WP_001251224.1	glucosamine-6-phosphate isomerase	Antitoxin
E3S98_RS00775	122118..122270	+	153	WP_000031108.1	hypothetical protein	Toxin
E3S98_RS00780	122932..123327	+	396	WP_045174101.1	hypothetical protein	-
E3S98_RS00785	123523..124908	+	1386	WP_045174099.1	class II fumarate hydratase	-
E3S98_RS00790	125326..126147	-	822	WP_045174097.1	RluA family pseudouridine synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Glucosamine-6-phosphate isomerase (124-196)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5936.28 Da
• Isoelectric Point: 3.8962

>T147673 WP_000031108.1 NZ_CP047786:122118-122270 [Staphylococcus aureus]
MSKDKDPKLNYHEEENSMVTDFEDLKELGKEMEQISDQNDQEKNSEEDSQ

Nucleotide

• Length: 153 bp

>T147673 NZ_CP063213:44595-44966 [Trueperella sp. 19M2397]
ATGATTGTGGATACGTCCGCCCTGGTCGCAGTGGCATATAGGGAGCCCGGATACGAAAGGATCACCGACCTTCTTTTCGC
AAGGAACAGGTCAAAGATGTCGGTGGCCAGCGTCCTTGAGCTTCAACTCGTATTGCGATCTTCCGAATTGGCGGACGAAC
TCATCGAGATTTTCAATGTGGATCCGGTGGGGATTGATGTCGACCAGCTACGCGTGGCGCGGCACGCGGCGCAGCGATTC
GGAAAGGGGACCGGCTCAAAGGCGGGGCTGAACTACGGCGACTGTTTCTCCTACGCCCTCGCGATGACTGCCGGAGAGCC
GTTGTTGTTTGTGGGCGAGGATTTCACGCACACGGACGTCGTCGTTGCGTGA

Antitoxin

Protein

• Length: 200 a.a.
• Molecular weight: 22343.47 Da
• Isoelectric Point: 5.1445

>AT147673 WP_001251224.1 NZ_CP047786:121494-122093 [Staphylococcus aureus]
MAMNFKVFDNSQLVAEYAADIIRKQFNNNPTTIAGFHLDTDQAPVLDELKKNVEKHAVDFSQINILDYDDKKSYFEALGV
PAGQVYPIAYEKDAIELIADKIKTKENKGKLTLQVVSIDEQGKLNVSIRQGLMEAREIFLVVTGANKRDVVEKLYQENGK
TSFEPADLKAHRMVNVILDKEAAAGLPEDVKAYFTSRFA

Nucleotide

• Length: 600 bp

>AT147673 NZ_CP063213:44356-44598 [Trueperella sp. 19M2397]
ATGCCAACCATGAACATCAAAGATGAAGACGTGTACAACGCAGCTCGCCAGCTTGCACAGTTGCGTGGAACGTCGATGAC
TGGTGCCGTGCGTTCTGCCGTGGAAGAGGCTCTGGATGCCGAGATCAAGCGGCGCGAACGCGGGCGCTTGCGCCTTGCCG
AACTCCAAGACCGCATCGCGCAGGCCGCCGAGCCCCGCTTAGACGAGTCTGACCTGTATGACGATAGGGGAATGCCCCGA
TGA

Structures

Toxin

• AlphaFold DB: X5E2E6

Antitoxin

• AlphaFold DB: W8U4V4