Detailed information of TA system TA147669

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2700266..2700795
• Replicon: chromosome
• Accession: NZ_CP047785
• Organism: Staphylococcus aureus strain UP_1186

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: E3S99_RS13380
• Protein ID: WP_000621175.1
• Coordinates: 2700433..2700795 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: E3S99_RS13375
• Protein ID: WP_000948331.1
• Coordinates: 2700266..2700436 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3S99_RS13345	2695303..2695863	+	561	WP_001092406.1	K(+)-transporting ATPase subunit C	-
E3S99_RS13350	2696071..2696550	+	480	WP_001287077.1	hypothetical protein	-
E3S99_RS13355	2696543..2698126	+	1584	WP_001294637.1	PH domain-containing protein	-
E3S99_RS13360	2698113..2698604	+	492	WP_001205907.1	PH domain-containing protein	-
E3S99_RS13365	2698608..2698967	+	360	WP_000581199.1	holo-ACP synthase	-
E3S99_RS13370	2699033..2700181	+	1149	WP_160199839.1	alanine racemase	-
E3S99_RS13375	2700266..2700436	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
E3S99_RS13380	2700433..2700795	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
E3S99_RS13385	2701144..2702145	+	1002	WP_160199840.1	PP2C family protein-serine/threonine phosphatase	-
E3S99_RS13390	2702264..2702590	+	327	WP_001052491.1	anti-sigma factor antagonist	-
E3S99_RS13395	2702592..2703071	+	480	WP_001190825.1	anti-sigma B factor RsbW	-
E3S99_RS13400	2703046..2703816	+	771	WP_001041107.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T147669 WP_000621175.1 NZ_CP047785:2700433-2700795 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T147669 NZ_CP063202:2312711-2313004 [Erythrobacter sp. SCSIO 43205]
ATGACCCGCAAAGTCGTCTTTCGCACTGCCGCTAAACGGGACTTGTCTCAGCTTGGCGCGCAATCGGCACGAGAATGGGG
CGCCCGTCGAACACGGGGCTATCTTCTGGACCTAGATGCAAAGATTCAGAAGCTGGCTGAGAACCCGATGCTTGGGCATG
AGGCAGGACTGGGCCGCGCAGGTGTCCGGCGCATGAACAGCGGGCGACACGCTGTGTTCTATGTGGTTGGTGAGGAGCGC
ATCGAGATCGTGCGGATATTGCACGTTTCGAGAGATGCTGGGCGTTGGGTTTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT147669 WP_000948331.1 NZ_CP047785:2700266-2700436 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT147669 NZ_CP063202:2312475-2312714 [Erythrobacter sp. SCSIO 43205]
ATGGGAAAGAATACATCCGTTTCGCTTGGCGAACATTTCGAGGAATACACGCGCAGCAAGGTCGCAACGGGCGAGTATAG
CTCGACCAGCGAAGTCATTCGCGAAGCCTTGCGCCTGCACGAAGAACGCTCGGTCTTCAAATCCAAGCTGCTCGAAGCGA
TTGATGAAGGGCTCGCGAGCCCGATTGATGAGAGCTTCGATATGGATGCGTGGATCAACGAAACTTTCCCAGAGGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1