Detailed information of TA system TA147651

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2015943..2016472
• Replicon: chromosome
• Accession: NZ_CP047784
• Organism: Staphylococcus aureus strain UP_1209

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: E3T00_RS09820
• Protein ID: WP_000621175.1
• Coordinates: 2015943..2016305 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: E3T00_RS09825
• Protein ID: WP_000948331.1
• Coordinates: 2016302..2016472 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3T00_RS09800	2012922..2013692	-	771	WP_001041107.1	RNA polymerase sigma factor SigB	-
E3T00_RS09805	2013667..2014146	-	480	WP_001190830.1	anti-sigma B factor RsbW	-
E3T00_RS09810	2014148..2014474	-	327	WP_001052491.1	anti-sigma factor antagonist	-
E3T00_RS09815	2014593..2015594	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
E3T00_RS09820	2015943..2016305	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
E3T00_RS09825	2016302..2016472	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
E3T00_RS09830	2016557..2017705	-	1149	WP_001281145.1	alanine racemase	-
E3T00_RS09835	2017771..2018130	-	360	WP_000581197.1	holo-ACP synthase	-
E3T00_RS09840	2018134..2018625	-	492	WP_072353916.1	PH domain-containing protein	-
E3T00_RS09845	2018612..2020195	-	1584	WP_043044023.1	PH domain-containing protein	-
E3T00_RS09850	2020188..2020667	-	480	WP_001287078.1	hypothetical protein	-
E3T00_RS09855	2020876..2021436	-	561	WP_001092409.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T147651 WP_000621175.1 NZ_CP047784:c2016305-2015943 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T147651 NZ_CP063166:c4041975-4041118 [Variovorax paradoxus]
ATGCAGACCACCGACATCCAGGCCTCGATGCCGCCCGCTTCCGAGCTGGCGCGGCTCGACGCGCGCTTCGCGGCCCGCGA
GGTCGCGGTCGCGGGCGGTGCCGTGGTGTCGGTGCGCGAACGCGGGCAGGGCCCGGCGATCGTCTGCCTGCATGGCATCG
GCTCGGGCGCGGCCTCGTGGCTCGAGGTCGCCGAGCTGCTCGCGCCCGAGGCGCGCCTGATCGCCTGGGACGCCCCGGGC
TACGGCGCATCGACGCCGCTCGAGGCCGATGCACCGACGGCCGCCGACTACGCGGCGCGCCTGAGTGCCTTGCTCGATGC
GCTGGCGATCGACAGCTGCCTGCTGCTCGGCCATTCGCTCGGCGCGATCGTCGCCGCCGCGGCCGCGCGCCGCGATGCGC
GCATCCGCCGGCTGCTGCTGGTCAGCCCCGCGATCGGCTACGGCGCCGCGCAGCGCGCGGAGGCGCGCGCCAAGGTGCGT
GCCGAACGGCTCGCCACGCTAGACCAGCTCGGCATCGCCGGCATGGCCGCGCAGCGCGCGGGCCGGTTGCTGTCCGACAA
CGCTAGCGAGCCCGCGCGCCAATGGGTGCGCTGGAACATGGCGCGCCTCAACGACCGCGGCTACCGCCAGGCCGTCGAGC
TGCTCTGCAACGCCGACCTGCTGGCCGAGCTGCCGCCGCCGATGCCGGTGCGCGTGGCCTGCGGCGCGCTCGATGCCGTC
ACGCCGCCCGCGGCCTGCGAGGACGTGGCGCGGCGCTGCGGCGTGGCGCTCGAATTGGTGGACGATGCGGGCCACGCCGG
CTACGTGGAGCAACCGCGGGCCGTCGCCGCGCTGCTGCGCGAATCGCTCGCCCGCTGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT147651 WP_000948331.1 NZ_CP047784:c2016472-2016302 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT147651 NZ_CP063166:c4041077-4040241 [Variovorax paradoxus]
ATGGCCACCAACGACAACGACGCGATGGAAGAGGGCGCGGACCGCTACAACGTGCCCGCGCTCGAGCGCGGACTGCGCGT
GCTGTGCGAGTTCAGCCGCGAGAGCCGCACGCTCTCGGCGCCCGAGCTGGCGCGCCGCTTCGACCTGCCGCGCTCGACCG
TGTTCCGCCTGCTCACCACGCTCGAGAACATGGGCTTTCTCGAGCGCGCCGAGGGTGGGCGCGACTACCGCCTGGGCCTG
GCCGTGCTGCGCCTGGGCTTCGAATACCTGGCCTCGCTCGAGCTCACGCAGCTCGGCACGCCGCTGCTCAACCGCCTCTG
CGAGGAGCTGCGCACGCCCTGCAACCTGGTGGTGCGCGATGGCCGCTCGATCGTCTACGTCGCCAAGGTCGCGCCGCCCA
CGCCCTTCGCGAGCTCGGTCACCGTGGGCACGCGGCTGCCGGCCCATGCCACGGTGCTGGGCCGCATCCTGCTGGAGGAC
CTGACTCTGCCGCAGCTGCGCGCCCTCTATCCCGAGGACAAGCTCGAGACCTTCTCGCCGAGCACGCCGAAGACCGTCAA
CGAGCTGTTCGACATGGTGCAGGCCGACCGCCAGCGCGGCTACGTGCTCGGCGAGGGCTTCTTCGAATCGAACATCTCGA
CCATCGCCGCGCCGGTGCGCGACCACGGCGGCCACATCGTGGCGGCGCTGGGCGCCACCATCACCTCGGGCCACATCGAC
GAGAACCGCATGGACGAGATGGTGCAGCGCGTGCGCGGCACGGCCGACGAGATCTCGGGCCTGCTCAACTATTCGCCCGC
GCGCGCCGCCAAGGTGGTGCCGCTGCGCAGCGCCTGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1