Detailed information of TA system TA147642

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2036891..2037420
• Replicon: chromosome
• Accession: NZ_CP047783
• Organism: Staphylococcus aureus strain UP_1313

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: E3T03_RS09865
• Protein ID: WP_000621175.1
• Coordinates: 2036891..2037253 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: E3T03_RS09870
• Protein ID: WP_000948331.1
• Coordinates: 2037250..2037420 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3T03_RS09845	2033870..2034640	-	771	WP_001041107.1	RNA polymerase sigma factor SigB	-
E3T03_RS09850	2034615..2035094	-	480	WP_001190825.1	anti-sigma B factor RsbW	-
E3T03_RS09855	2035096..2035422	-	327	WP_001052491.1	anti-sigma factor antagonist	-
E3T03_RS09860	2035541..2036542	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
E3T03_RS09865	2036891..2037253	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
E3T03_RS09870	2037250..2037420	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
E3T03_RS09875	2037505..2038653	-	1149	WP_001280702.1	alanine racemase	-
E3T03_RS09880	2038719..2039078	-	360	WP_000581194.1	holo-ACP synthase	-
E3T03_RS09885	2039082..2039573	-	492	WP_001205915.1	PH domain-containing protein	-
E3T03_RS09890	2039560..2041143	-	1584	WP_001294642.1	PH domain-containing protein	-
E3T03_RS09895	2041136..2041615	-	480	WP_001287083.1	hypothetical protein	-
E3T03_RS09900	2041824..2042384	-	561	WP_001092405.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T147642 WP_000621175.1 NZ_CP047783:c2037253-2036891 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T147642 NZ_CP063158:245049-245399 [Bacillus cereus]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTATGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAACGCTTAACGGACAAAATCACTCATTTAGATGAAGTGATGATGAGTCGTGTAGATGAAGCGTT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT147642 WP_000948331.1 NZ_CP047783:c2037420-2037250 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT147642 NZ_CP063158:244757-245044 [Bacillus cereus]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAATTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTATATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCG
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1