Detailed information of TA system TA147538

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2732142..2732671
• Replicon: chromosome
• Accession: NZ_CP047777
• Organism: Staphylococcus aureus strain UP_1632

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: E3T16_RS13620
• Protein ID: WP_000621175.1
• Coordinates: 2732309..2732671 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: E3T16_RS13615
• Protein ID: WP_000948331.1
• Coordinates: 2732142..2732312 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3T16_RS13585	2727179..2727739	+	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-
E3T16_RS13590	2727947..2728426	+	480	WP_001287087.1	hypothetical protein	-
E3T16_RS13595	2728419..2730002	+	1584	WP_001294626.1	PH domain-containing protein	-
E3T16_RS13600	2729989..2730480	+	492	WP_001205912.1	PH domain-containing protein	-
E3T16_RS13605	2730484..2730843	+	360	WP_000581197.1	holo-ACP synthase	-
E3T16_RS13610	2730909..2732057	+	1149	WP_001281154.1	alanine racemase	-
E3T16_RS13615	2732142..2732312	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
E3T16_RS13620	2732309..2732671	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
E3T16_RS13625	2733021..2734022	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
E3T16_RS13630	2734141..2734467	+	327	WP_001052491.1	anti-sigma factor antagonist	-
E3T16_RS13635	2734469..2734948	+	480	WP_001190829.1	anti-sigma B factor RsbW	-
E3T16_RS13640	2734923..2735693	+	771	WP_001041111.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T147538 WP_000621175.1 NZ_CP047777:2732309-2732671 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T147538 NZ_CP063046:c1216212-1215898 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT147538 WP_000948331.1 NZ_CP047777:2732142-2732312 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT147538 NZ_CP063046:c1216448-1216215 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1