Detailed information of TA system TA147515

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 90883..91409
• Replicon: plasmid pEcl1-1
• Accession: NZ_CP047766
• Organism: Enterobacter hormaechei strain Eho-1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1EXL1
• Locus tag: GVI62_RS22745
• Protein ID: WP_000323025.1
• Coordinates: 91122..91409 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: J5W3H0
• Locus tag: GVI62_RS22740
• Protein ID: WP_004196370.1
• Coordinates: 90883..91122 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GVI62_RS22715	86151..87527	+	1377	WP_004196363.1	chromate efflux transporter	-
GVI62_RS22720	87558..88247	-	690	WP_013087175.1	hypothetical protein	-
GVI62_RS22725	88261..88998	-	738	WP_008460272.1	HupE/UreJ family protein	-
GVI62_RS22730	89042..89407	-	366	WP_009651956.1	hypothetical protein	-
GVI62_RS22740	90883..91122	+	240	WP_004196370.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
GVI62_RS22745	91122..91409	+	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
GVI62_RS22750	91476..92510	-	1035	Protein_109	IS481 family transposase	-
GVI62_RS22755	92582..92740	+	159	WP_013087178.1	type I toxin-antitoxin system Hok family toxin	-
GVI62_RS22760	93616..93930	+	315	WP_044157807.1	hypothetical protein	-
GVI62_RS22765	93991..94452	+	462	WP_013087181.1	hypothetical protein	-
GVI62_RS22770	94540..94740	+	201	WP_013087182.1	hypothetical protein	-
GVI62_RS22775	94781..95266	+	486	Protein_114	SAM-dependent DNA methyltransferase	-
GVI62_RS22780	95267..95890	+	624	Protein_115	conjugal transfer protein TraI	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	dfrA14 / qnrB1 / aac(6')-Ib-cr / blaOXA-1 / aac(3)-IIa / blaVIM-1 / ant(3'')-Ia / qacE / sul1 / qnrB56	-	1..178304	178304
	-	inside	IScluster/Tn	blaVIM-1 / ant(3'')-Ia / qacE / sul1 / qnrB56	-	89443..125564	36121

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (3-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T147515 WP_000323025.1 NZ_CP047766:91122-91409 [Enterobacter hormaechei]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T147515 NZ_CP063019:360117-360464 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTCCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTTTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGCGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9018.43 Da
• Isoelectric Point: 4.3894

>AT147515 WP_004196370.1 NZ_CP047766:90883-91122 [Enterobacter hormaechei]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLCNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT147515 NZ_CP063019:360464-360763 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGAAAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGACTGATAACGACCCTCGGCTTTCGACCCTAAAAAGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Structures

Toxin

• PDB: 4V7K
• PDB: 4V7J
• PDB: 4FXI
• PDB: 4FXE
• PDB: 2KC8
• PDB: 4FXH
• PDB: 2KC9

Antitoxin

• AlphaFold DB: A0A2J4QRH4