Detailed information of TA system TA147498

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 97745..98412
• Replicon: chromosome
• Accession: NZ_CP047765
• Organism: Enterobacter hormaechei strain Eho-1

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: -
• Locus tag: GVI62_RS00510
• Protein ID: WP_060579732.1
• Coordinates: 97745..98065 (-)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: GVI62_RS00515
• Protein ID: WP_060579733.1
• Coordinates: 98086..98412 (-)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GVI62_RS00465	92759..93640	+	882	WP_032608178.1	helix-turn-helix domain-containing protein	-
GVI62_RS00470	93689..94132	+	444	WP_045346041.1	GNAT family N-acetyltransferase	-
GVI62_RS00475	94165..94686	-	522	WP_017383638.1	hypothetical protein	-
GVI62_RS00480	94749..95114	-	366	WP_072034771.1	DUF2778 domain-containing protein	-
GVI62_RS00485	95124..95609	-	486	WP_045895876.1	type VI secretion system tube protein Hcp	-
GVI62_RS00490	95986..96282	-	297	WP_023323949.1	SymE family type I addiction module toxin	-
GVI62_RS00495	96411..96581	+	171	WP_065186978.1	DUF3950 domain-containing protein	-
GVI62_RS00500	96587..97294	+	708	WP_126542334.1	hypothetical protein	-
GVI62_RS00505	97357..97599	+	243	WP_045894635.1	hypothetical protein	-
GVI62_RS00510	97745..98065	-	321	WP_060579732.1	TA system toxin CbtA family protein	Toxin
GVI62_RS00515	98086..98412	-	327	WP_060579733.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
GVI62_RS00520	98425..98895	-	471	WP_060579734.1	DNA repair protein RadC	-
GVI62_RS00525	98965..99786	-	822	WP_060579735.1	DUF945 domain-containing protein	-
GVI62_RS23375	100229..100420	-	192	WP_072199593.1	hypothetical protein	-
GVI62_RS00530	100479..101318	-	840	WP_060579736.1	hypothetical protein	-
GVI62_RS00535	101378..101812	-	435	WP_060579737.1	hypothetical protein	-
GVI62_RS00540	102121..103284	-	1164	WP_060579738.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	79222..116035	36813

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-98)

Antitoxin

• Antitoxin CbeA (7-107)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12078.85 Da
• Isoelectric Point: 4.7440

>T147498 WP_060579732.1 NZ_CP047765:c98065-97745 [Enterobacter hormaechei]
MNIQPATTPQAAKPCLSPLAVWQMLLASLLDQHYGLTLNDTPFSDETVIQKHIEAGITLADAVNFLVERYELVRIDQKGY
SVQDQEPWLTYMDVHRAQLKLNVERL

Nucleotide

• Length: 321 bp

>T147498 NZ_CP063008:4178296-4178514 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 12187.90 Da
• Isoelectric Point: 6.4729

>AT147498 WP_060579733.1 NZ_CP047765:c98412-98086 [Enterobacter hormaechei]
MSLIPAHEWGLKSDIVPRFGARLVQEGNRLHFLADRASLMGNFSDTECFKLNDAFPHFISQMESMLTTGELIPCHHRCVT
LYHNGFTCEADTLGSCGYVYIAVYSTQR

Nucleotide

• Length: 327 bp

>AT147498 NZ_CP063008:4177895-4178269 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG