Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 29277..29920 Replicon plasmid pEcl4-5
Accession NZ_CP047745
Organism Enterobacter hormaechei strain Eho-4

Toxin (Protein)


Gene name vagD Uniprot ID Q84A06
Locus tag GVI65_RS26910 Protein ID WP_000754566.1
Coordinates 29504..29920 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag GVI65_RS26905 Protein ID WP_001261276.1
Coordinates 29277..29507 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GVI65_RS26875 24913..25779 - 867 WP_004118283.1 replication initiation protein -
GVI65_RS26880 26309..26413 + 105 WP_032409716.1 hypothetical protein -
GVI65_RS26885 26542..26799 + 258 WP_000764642.1 hypothetical protein -
GVI65_RS26890 26857..27633 - 777 WP_020956881.1 site-specific integrase -
GVI65_RS26895 27645..28331 - 687 WP_020956882.1 hypothetical protein -
GVI65_RS26900 28395..28688 - 294 WP_020956883.1 hypothetical protein -
GVI65_RS26905 29277..29507 + 231 WP_001261276.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
GVI65_RS26910 29504..29920 + 417 WP_000754566.1 type II toxin-antitoxin system VapC family toxin Toxin
GVI65_RS26915 30001..30705 + 705 WP_001067855.1 IS6-like element IS26 family transposase -
GVI65_RS26920 30827..31732 + 906 WP_000219391.1 Mph(A) family macrolide 2'-phosphotransferase -
GVI65_RS26925 31729..32967 + 1239 WP_000004159.1 MFS transporter -
GVI65_RS26930 32967..33551 + 585 WP_001137892.1 TetR family transcriptional regulator -
GVI65_RS26935 34044..34808 - 765 WP_197347221.1 IS6 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid qacE / ant(3'')-Ia / blaVIM-1 / tet(A) / mph(A) - 1..37460 37460


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15006.52 Da        Isoelectric Point: 9.2957

>T147479 WP_000754566.1 NZ_CP047745:29504-29920 [Enterobacter hormaechei]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFARVPGLVLEDWVK

Download         Length: 417 bp

>T147479 NZ_CP062998:c68890-68603 [Klebsiella pneumoniae]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT147479 WP_001261276.1 NZ_CP047745:29277-29507 [Enterobacter hormaechei]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT147479 NZ_CP062998:c69129-68890 [Klebsiella pneumoniae]
ATGGGTAGCATTAACCTACGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGCGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGACTTTGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A387K1G3

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References