Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 115915..117538 Replicon plasmid pEcl4-1
Accession NZ_CP047741
Organism Enterobacter hormaechei strain Eho-4

Toxin (Protein)


Gene name hipA Uniprot ID C1IUI3
Locus tag GVI65_RS24050 Protein ID WP_000088044.1
Coordinates 116213..117538 (+) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A6M3PBR7
Locus tag GVI65_RS24045 Protein ID WP_012477377.1
Coordinates 115915..116208 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GVI65_RS24025 111714..112106 + 393 WP_000341066.1 hypothetical protein -
GVI65_RS24030 112765..113421 + 657 WP_000462754.1 trypsin-like serine protease -
GVI65_RS24035 113624..114121 + 498 WP_000340139.1 membrane protein -
GVI65_RS24040 114126..115514 + 1389 WP_000083579.1 hypothetical protein -
GVI65_RS24045 115915..116208 + 294 WP_012477377.1 helix-turn-helix transcriptional regulator Antitoxin
GVI65_RS24050 116213..117538 + 1326 WP_000088044.1 type II toxin-antitoxin system HipA family toxin Toxin
GVI65_RS24055 117599..117805 + 207 WP_000134171.1 hypothetical protein -
GVI65_RS24060 117906..118316 + 411 WP_000985909.1 hypothetical protein -
GVI65_RS24065 118329..119144 + 816 WP_001641519.1 HNH endonuclease -
GVI65_RS24070 119397..119822 + 426 WP_000278471.1 hypothetical protein -
GVI65_RS24075 120371..120679 + 309 WP_001224686.1 hypothetical protein -
GVI65_RS24080 120695..121552 + 858 WP_000784387.1 HNH endonuclease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaACC-1 / sul1 / aph(6)-Id / aph(3'')-Ib / mcr-9 / qacE / ant(3'')-Ia / ere(A) / aac(3)-IId / blaTEM-1B / catA1 - 1..344276 344276


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(8-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50333.32 Da        Isoelectric Point: 6.5044

>T147478 WP_000088044.1 NZ_CP047741:116213-117538 [Enterobacter hormaechei]
MSRKQQRLAIWMNGIKVGYWEKSKGVDSLQYLPDWSADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMRSSVENEWLCSVLLEQYGLPVARTQIAHFEDQKALVVERF
DRKWSGNGQWIIRLPQEDMCQALGISPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIVFWMMAATDGHAKNFS
ISIGPQGRYHLTPLYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

>T147478 NZ_CP062998:17739-18155 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGAATCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGACCGCATTGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCTATCGCGGCCGGCGCCATCCTGGTGACGAATAATACAAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10860.30 Da        Isoelectric Point: 10.3134

>AT147478 WP_012477377.1 NZ_CP047741:115915-116208 [Enterobacter hormaechei]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSTTASSEGK
QTEEMLKSNSPARQEKW

Download         Length: 294 bp

>AT147478 NZ_CP062998:17512-17742 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATTCGGCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2S1XXW1

Antitoxin

Source ID Structure
AlphaFold DB A0A6M3PBR7

References