Detailed information of TA system TA147477

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 4775778..4776394
• Replicon: chromosome
• Accession: NZ_CP047740
• Organism: Enterobacter hormaechei strain Eho-4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: GVI65_RS23095
• Protein ID: WP_015569913.1
• Coordinates: 4775778..4776149 (-)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A6G4MTK3
• Locus tag: GVI65_RS23100
• Protein ID: WP_015569912.1
• Coordinates: 4776152..4776394 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GVI65_RS23080	4773278..4774180	+	903	WP_014072386.1	formate dehydrogenase subunit beta	-
GVI65_RS23085	4774177..4774812	+	636	WP_015569914.1	formate dehydrogenase cytochrome b556 subunit	-
GVI65_RS23090	4774809..4775738	+	930	WP_003861956.1	formate dehydrogenase accessory protein FdhE	-
GVI65_RS23095	4775778..4776149	-	372	WP_015569913.1	PIN domain-containing protein	Toxin
GVI65_RS23100	4776152..4776394	-	243	WP_015569912.1	ribbon-helix-helix domain-containing protein	Antitoxin
GVI65_RS23105	4776593..4777513	+	921	WP_015569911.1	alpha/beta hydrolase	-
GVI65_RS23110	4777522..4778463	-	942	WP_015569910.1	fatty acid biosynthesis protein FabY	-
GVI65_RS23115	4778508..4778945	-	438	WP_015569909.1	D-tyrosyl-tRNA(Tyr) deacylase	-
GVI65_RS23120	4778942..4779823	-	882	WP_003861949.1	virulence factor BrkB family protein	-
GVI65_RS23125	4779817..4780416	-	600	WP_003861947.1	glucose-1-phosphatase	-
GVI65_RS23130	4780535..4781335	-	801	WP_003861944.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-115)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-49)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13767.90 Da
• Isoelectric Point: 6.4882

>T147477 WP_015569913.1 NZ_CP047740:c4776149-4775778 [Enterobacter hormaechei]
MEHMAVFDTNILIDLFNNRIEAADAIEHTASHRAISLITWMEVMVSARRHGHEAKTAAVMGAFEIIDVSRDIAERSVLLR
EKHGMKLPDAIILATAQSRKCPLISRNTKDFAGIDEVLTPYQV

Nucleotide

• Length: 372 bp

>T147477 NZ_CP062998:c16695-16390 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGGTCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTCTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGACGACAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGGCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9440.72 Da
• Isoelectric Point: 4.4076

>AT147477 WP_015569912.1 NZ_CP047740:c4776394-4776152 [Enterobacter hormaechei]
MSVLAELDMGRILIDLSDDVIQRLDNLKQLRNQPRAELLREAIELYLDQQSSSVIRNALGLWGDRKEDGLEYERKLREEW

Nucleotide

• Length: 243 bp

>AT147477 NZ_CP062998:c16915-16697 [Klebsiella pneumoniae]
ATGAAGCAGCGCATCACCGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCGGACGTCAATATCTCCGGCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCCGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A6G4MTK3