Detailed information of TA system TA147461

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 17538..18181
• Replicon: plasmid pEcl5-2
• Accession: NZ_CP047738
• Organism: Enterobacter hormaechei strain Eho-5

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q84A06
• Locus tag: GVI66_RS25120
• Protein ID: WP_000754566.1
• Coordinates: 17538..17954 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q84A07
• Locus tag: GVI66_RS25125
• Protein ID: WP_001261276.1
• Coordinates: 17951..18181 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GVI66_RS25095	12650..13414	+	765	WP_197347221.1	IS6 family transposase	-
GVI66_RS25100	13907..14491	-	585	WP_001137892.1	TetR family transcriptional regulator	-
GVI66_RS25105	14491..15729	-	1239	WP_000004159.1	MFS transporter	-
GVI66_RS25110	15726..16631	-	906	WP_000219391.1	Mph(A) family macrolide 2'-phosphotransferase	-
GVI66_RS25115	16753..17457	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
GVI66_RS25120	17538..17954	-	417	WP_000754566.1	type II toxin-antitoxin system VapC family toxin	Toxin
GVI66_RS25125	17951..18181	-	231	WP_001261276.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
GVI66_RS25130	18770..19063	+	294	WP_020956883.1	hypothetical protein	-
GVI66_RS25135	19127..19813	+	687	WP_020956882.1	hypothetical protein	-
GVI66_RS25140	19825..20601	+	777	WP_020956881.1	site-specific integrase	-
GVI66_RS25145	20659..20916	-	258	WP_000764642.1	hypothetical protein	-
GVI66_RS25150	21045..21149	-	105	WP_032409716.1	hypothetical protein	-
GVI66_RS25155	21679..22545	+	867	WP_004118283.1	replication initiation protein	-
GVI66_RS25160	22722..22991	-	270	WP_000339857.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	tet(A) / blaVIM-1 / ant(3'')-Ia / qacE / sul1 / mph(A)	-	1..37715	37715

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15006.52 Da
• Isoelectric Point: 9.2957

>T147461 WP_000754566.1 NZ_CP047738:c17954-17538 [Enterobacter hormaechei]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFARVPGLVLEDWVK

Nucleotide

• Length: 417 bp

>T147461 NZ_CP062987:c83022-82540 [Klebsiella pneumoniae]
ATGGGAATGAGAGCGCCTGAATCCCTGACGGCTGAACATAACATCGCTGAGTTCTGCTGCCAGGAGCCTGCCCTTAATGA
GTGGTTAAAGAAGAAGGCTCTCAGAAACCACAGTACCGGCATTTCCCGTGTCTATGTCGTCTGCGCAGAAAACACTAACC
GGGTCATCGGTTATTATTGTTTATCTTCGGGTAGCGTCCACCGTAACACAGTGCCTGGGGCCTATAGACGCAACGCGCCT
GATGTCATTCCAGTCATTGTTTTGGGACGGCTTGCCATCGACCAGGCCTGGGCCGGGAATGGGCTTGGTGCCGCACTTCT
TAAGGATGCTATATACCGTACGCAAAACATAGCCTTTCAGGTCGGGGTGAGAGCACTGGCCGTGCATGCGCTGAATGAAG
AAGTTAAATGTTTTTACACCCGTTTTGGCTTTGAGCCTTCCATAGTCAACACGCTGACATTGTTATTCCCGATAAAAGTC
TGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8721.74 Da
• Isoelectric Point: 4.3533

>AT147461 WP_001261276.1 NZ_CP047738:c18181-17951 [Enterobacter hormaechei]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT147461 NZ_CP062987:c83291-83013 [Klebsiella pneumoniae]
ATGAAACCAGACGTTCGTGAAGCTCCGATTAATATCCGGGCCAAAACATCACAGCGTGACCTGATCGACATGGCCGCAAG
CCTGGTCTCAAAATCACGGACTGATTTTATGCTTGAGGTTGCCTGCAGGGAGGCACAGGACATTCTGCTTGATCAACGCC
TGTTCCTCCTCAATGACAATCAGTTTGAGGCGTTTATTGAAGAGCTTGACGCCCCAATAACGCCGGAGCGCCAGGCGCGA
ATCGATAACCTGATGAACCGCAAATCACCATGGGAATGA

Structures

Toxin

• AlphaFold DB: A0A387K1G3

Antitoxin

• AlphaFold DB: A0A387K023