Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 165936..167559 Replicon plasmid pEcl5-1
Accession NZ_CP047737
Organism Enterobacter hormaechei strain Eho-5

Toxin (Protein)


Gene name hipA Uniprot ID C1IUI3
Locus tag GVI66_RS24255 Protein ID WP_000088044.1
Coordinates 166234..167559 (+) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A6M3PBR7
Locus tag GVI66_RS24250 Protein ID WP_012477377.1
Coordinates 165936..166229 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GVI66_RS24230 161735..162127 + 393 WP_000341066.1 hypothetical protein -
GVI66_RS24235 162786..163442 + 657 WP_000462754.1 trypsin-like serine protease -
GVI66_RS24240 163645..164142 + 498 WP_000340139.1 membrane protein -
GVI66_RS24245 164147..165535 + 1389 WP_000083579.1 hypothetical protein -
GVI66_RS24250 165936..166229 + 294 WP_012477377.1 helix-turn-helix transcriptional regulator Antitoxin
GVI66_RS24255 166234..167559 + 1326 WP_000088044.1 type II toxin-antitoxin system HipA family toxin Toxin
GVI66_RS24260 167620..167826 + 207 WP_000134171.1 hypothetical protein -
GVI66_RS24265 167927..168337 + 411 WP_000985909.1 hypothetical protein -
GVI66_RS24270 168350..169165 + 816 WP_001641519.1 HNH endonuclease -
GVI66_RS24275 169418..169843 + 426 WP_000278471.1 hypothetical protein -
GVI66_RS24280 170392..170700 + 309 WP_001224686.1 hypothetical protein -
GVI66_RS24285 170716..171573 + 858 WP_000784387.1 HNH endonuclease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaACC-1 / dfrA14 / qnrS1 / sul1 / aph(6)-Id / aph(3'')-Ib / mcr-9 / qacE / aadA2 / dfrA12 / aac(3)-IId / blaTEM-1B / catA1 - 1..310486 310486


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(8-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50333.32 Da        Isoelectric Point: 6.5044

>T147460 WP_000088044.1 NZ_CP047737:166234-167559 [Enterobacter hormaechei]
MSRKQQRLAIWMNGIKVGYWEKSKGVDSLQYLPDWSADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMRSSVENEWLCSVLLEQYGLPVARTQIAHFEDQKALVVERF
DRKWSGNGQWIIRLPQEDMCQALGISPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIVFWMMAATDGHAKNFS
ISIGPQGRYHLTPLYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

>T147460 NZ_CP062986:c5250892-5250509 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10860.30 Da        Isoelectric Point: 10.3134

>AT147460 WP_012477377.1 NZ_CP047737:165936-166229 [Enterobacter hormaechei]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSTTASSEGK
QTEEMLKSNSPARQEKW

Download         Length: 294 bp

>AT147460 NZ_CP062986:c5251134-5250892 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2S1XXW1

Antitoxin

Source ID Structure
AlphaFold DB A0A6M3PBR7

References