Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 2827729..2828334 Replicon chromosome
Accession NZ_CP047736
Organism Enterobacter hormaechei strain Eho-5

Toxin (Protein)


Gene name hicA Uniprot ID D2TUP9
Locus tag GVI66_RS13555 Protein ID WP_012906750.1
Coordinates 2827729..2827908 (+) Length 60 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A822X3T2
Locus tag GVI66_RS13560 Protein ID WP_032619507.1
Coordinates 2827930..2828334 (+) Length 135 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GVI66_RS13530 2823058..2823774 + 717 WP_032619512.1 ABC transporter ATP-binding protein -
GVI66_RS13535 2823940..2824410 + 471 WP_017384432.1 hypothetical protein -
GVI66_RS13540 2824416..2825342 - 927 WP_032619511.1 LysR family transcriptional regulator -
GVI66_RS13545 2825372..2825695 - 324 WP_017384434.1 hypothetical protein -
GVI66_RS13550 2825698..2826453 - 756 Protein_2652 DUF4225 domain-containing protein -
GVI66_RS13555 2827729..2827908 + 180 WP_012906750.1 type II toxin-antitoxin system HicA family toxin Toxin
GVI66_RS13560 2827930..2828334 + 405 WP_032619507.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
GVI66_RS13565 2828375..2829445 - 1071 WP_032635096.1 glycosyltransferase family 9 protein -
GVI66_RS13570 2829522..2830100 - 579 WP_032619505.1 tail fiber assembly protein -
GVI66_RS13575 2830100..2832277 - 2178 WP_058670599.1 phage tail protein -
GVI66_RS13580 2832280..2832831 - 552 WP_032619504.1 phage tail protein I -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - fliA 2823070..2932796 109726


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(4-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-126)


Sequences

Toxin        


Download         Length: 60 a.a.        Molecular weight: 6644.85 Da        Isoelectric Point: 10.9132

>T147455 WP_012906750.1 NZ_CP047736:2827729-2827908 [Enterobacter hormaechei]
MDSRNAIAMIEADGWYLVRVKGSHHQFKHPTKKGLVTVKHPQKDIPLPTLKSIKKQAGL

Download         Length: 180 bp

>T147455 NZ_CP062986:c3174682-3174506 [Klebsiella pneumoniae]
GTGAAGCAAAGCGAGTTGAGGCGTTGGCTTGCAGCTCAAGGGGCAGAATTTAAAGATGGTACTAACCACTTGAAAATCTA
TCTCAACGGCAAGCAAACGGTAATGCCGAGACATCCGGGGAAGGAAATACCGGAACCGCTGAGGAAGGCAATTCTTAAGC
AACTCGGTATCAAATAA

Antitoxin


Download         Length: 135 a.a.        Molecular weight: 14676.53 Da        Isoelectric Point: 4.2136

>AT147455 WP_032619507.1 NZ_CP047736:2827930-2828334 [Enterobacter hormaechei]
MLYPVAIDKGDSSFGVRVPDIPGCFSGGEDYQDAIESVREAIEAHIELLVEDGESVPEATTVENWLSDPEYAGAVWALVD
VDITRLMGKAEKINVTLPSLLIRRIDQFVAAHPEYGSRSGFLSRVAADKVINQR

Download         Length: 405 bp

>AT147455 NZ_CP062986:c3174458-3174051 [Klebsiella pneumoniae]
ATGCGATACCCGGTAATTTTTGAGCATGACGAAACCGGCTGGGCGGTATTCTTTCCGGATATCCCAGAGGCAATGACGGG
AGGAGAGACCAGGGAGGAAGCGTTAGAAATGGCGCAAGATGCCCTGGTGACGGCGTTTGATTTTTACTTTGACGACCGCC
GGGAGATTCCCGCTCCATCAGCAGAAGGTGATGCGTTTGTTGAGGTTCCGGCCAGCGTGGCAGCTAAAGTATTGCTGTTA
AACCGTCTGGTCAGCACCAACACCAGCAATGCTGATCTCGCCCGCTTGATTAACACGCGCCCACAGGAAGTGCAGCGCAT
CGTGTCTCTTGGTCACAGTACCAAAATCGATACGATCCAAAAAGCGCTATCAGCGTTGGGGCAAAAGATGGAAATTGTCG
TCCACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7D6XDK4

Antitoxin

Source ID Structure
AlphaFold DB A0A822X3T2

References