Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 17127..17770 Replicon plasmid pEcl6-3
Accession NZ_CP047732
Organism Enterobacter hormaechei strain Eho-6

Toxin (Protein)


Gene name vagD Uniprot ID Q84A06
Locus tag GVI67_RS24170 Protein ID WP_000754566.1
Coordinates 17354..17770 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag GVI67_RS24165 Protein ID WP_001261276.1
Coordinates 17127..17357 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GVI67_RS24130 12317..12586 + 270 WP_000339857.1 hypothetical protein -
GVI67_RS24135 12763..13629 - 867 WP_004118283.1 replication initiation protein -
GVI67_RS24140 14159..14263 + 105 WP_032409716.1 hypothetical protein -
GVI67_RS24145 14392..14649 + 258 WP_000764642.1 hypothetical protein -
GVI67_RS24150 14707..15483 - 777 WP_020956881.1 site-specific integrase -
GVI67_RS24155 15495..16181 - 687 WP_020956882.1 hypothetical protein -
GVI67_RS24160 16245..16538 - 294 WP_020956883.1 hypothetical protein -
GVI67_RS24165 17127..17357 + 231 WP_001261276.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
GVI67_RS24170 17354..17770 + 417 WP_000754566.1 type II toxin-antitoxin system VapC family toxin Toxin
GVI67_RS24175 17851..18555 + 705 WP_001067855.1 IS6-like element IS26 family transposase -
GVI67_RS24180 18677..19582 + 906 WP_000219391.1 Mph(A) family macrolide 2'-phosphotransferase -
GVI67_RS24185 19579..20817 + 1239 WP_000004159.1 MFS transporter -
GVI67_RS24190 20817..21401 + 585 WP_001137892.1 TetR family transcriptional regulator -
GVI67_RS24195 21894..22658 - 765 WP_197347221.1 IS6 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid mph(A) / sul1 / qacE / ant(3'')-Ia / blaVIM-1 - 1..33171 33171


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15006.52 Da        Isoelectric Point: 9.2957

>T147442 WP_000754566.1 NZ_CP047732:17354-17770 [Enterobacter hormaechei]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFARVPGLVLEDWVK

Download         Length: 417 bp

>T147442 NZ_CP062985:c3065548-3065213 [Escherichia coli UTI89]
ATGGTAAGCCGATACGTACCCGATACGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCCGG
ACATCGTCCGGCTGTTGTCCTGAGTCCGTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTCTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTGCTGATTGGGTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT147442 WP_001261276.1 NZ_CP047732:17127-17357 [Enterobacter hormaechei]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT147442 NZ_CP062985:c3065796-3065548 [Escherichia coli UTI89]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGGGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A387K1G3

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References