Detailed information of TA system TA147441

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 119435..121058
• Replicon: plasmid pEcl6-1
• Accession: NZ_CP047730
• Organism: Enterobacter hormaechei strain Eho-6

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: C1IUI3
• Locus tag: GVI67_RS22490
• Protein ID: WP_000088044.1
• Coordinates: 119733..121058 (+)
• Length: 442 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A6M3PBR7
• Locus tag: GVI67_RS22485
• Protein ID: WP_012477377.1
• Coordinates: 119435..119728 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GVI67_RS22465	115234..115626	+	393	WP_000341066.1	hypothetical protein	-
GVI67_RS22470	116285..116941	+	657	WP_000462754.1	trypsin-like serine protease	-
GVI67_RS22475	117144..117641	+	498	WP_197347214.1	hypothetical protein	-
GVI67_RS22480	117646..119034	+	1389	WP_000083579.1	hypothetical protein	-
GVI67_RS22485	119435..119728	+	294	WP_012477377.1	helix-turn-helix transcriptional regulator	Antitoxin
GVI67_RS22490	119733..121058	+	1326	WP_000088044.1	type II toxin-antitoxin system HipA family toxin	Toxin
GVI67_RS22495	121119..121325	+	207	WP_000134171.1	hypothetical protein	-
GVI67_RS22500	121426..121836	+	411	WP_000985909.1	hypothetical protein	-
GVI67_RS22505	121849..122664	+	816	WP_001641519.1	HNH endonuclease	-
GVI67_RS22510	122917..123342	+	426	WP_000278471.1	hypothetical protein	-
GVI67_RS22515	123891..124199	+	309	WP_001224686.1	hypothetical protein	-
GVI67_RS22520	124215..125072	+	858	WP_000784387.1	HNH endonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A) / dfrA14 / qnrB1 / blaOXA-1 / aac(6')-Ib-cr / blaCTX-M-15 / blaTEM-1B / aph(6)-Id / aph(3'')-Ib / sul2 / ant(3'')-Ia / catA1	-	1..310659	310659

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (8-108)

Antitoxin

• Cro/C1-type helix-turn-helix domain (21-69)

Sequences

Toxin

Protein

• Length: 442 a.a.
• Molecular weight: 50333.32 Da
• Isoelectric Point: 6.5044

>T147441 WP_000088044.1 NZ_CP047730:119733-121058 [Enterobacter hormaechei]
MSRKQQRLAIWMNGIKVGYWEKSKGVDSLQYLPDWSADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMRSSVENEWLCSVLLEQYGLPVARTQIAHFEDQKALVVERF
DRKWSGNGQWIIRLPQEDMCQALGISPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIVFWMMAATDGHAKNFS
ISIGPQGRYHLTPLYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Nucleotide

• Length: 1326 bp

>T147441 NZ_CP062985:c2228120-2227869 [Escherichia coli UTI89]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10860.30 Da
• Isoelectric Point: 10.3134

>AT147441 WP_012477377.1 NZ_CP047730:119435-119728 [Enterobacter hormaechei]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSTTASSEGK
QTEEMLKSNSPARQEKW

Nucleotide

• Length: 294 bp

>AT147441 NZ_CP062985:c2227872-2227618 [Escherichia coli UTI89]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAAATTATCAAAGATACACGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Toxin

• AlphaFold DB: A0A2S1XXW1

Antitoxin

• AlphaFold DB: A0A6M3PBR7