Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 1684945..1685742 Replicon chromosome
Accession NZ_CP047729
Organism Enterobacter hormaechei strain Eho-6

Toxin (Protein)


Gene name KacT Uniprot ID F5RT91
Locus tag GVI67_RS08010 Protein ID WP_006809321.1
Coordinates 1685221..1685742 (+) Length 174 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID F5RT92
Locus tag GVI67_RS08005 Protein ID WP_001303459.1
Coordinates 1684945..1685214 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GVI67_RS07975 1680567..1681067 + 501 WP_032673548.1 DedA family protein -
GVI67_RS07980 1681172..1682413 + 1242 WP_006809325.1 bifunctional glucose-1-phosphatase/inositol phosphatase -
GVI67_RS07985 1682446..1682673 - 228 WP_006809324.1 YccJ family protein -
GVI67_RS07990 1682692..1683288 - 597 WP_006809323.1 NAD(P)H:quinone oxidoreductase -
GVI67_RS07995 1683679..1683849 + 171 WP_001273664.1 general stress protein -
GVI67_RS08000 1683966..1684871 + 906 WP_039273532.1 DMT family transporter -
GVI67_RS08005 1684945..1685214 + 270 WP_001303459.1 DUF1778 domain-containing protein Antitoxin
GVI67_RS08010 1685221..1685742 + 522 WP_006809321.1 GNAT family N-acetyltransferase Toxin
GVI67_RS08015 1685801..1687123 - 1323 WP_006809320.1 pyrimidine utilization transport protein G -
GVI67_RS08020 1687145..1687639 - 495 WP_006809319.1 pyrimidine utilization flavin reductase protein F -
GVI67_RS08025 1687649..1688239 - 591 WP_006809318.1 malonic semialdehyde reductase -
GVI67_RS08030 1688249..1689049 - 801 WP_006809317.1 pyrimidine utilization protein D -
GVI67_RS08035 1689057..1689443 - 387 WP_006809316.1 pyrimidine utilization protein C -
GVI67_RS08040 1689455..1690144 - 690 WP_006809315.1 pyrimidine utilization protein B -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(87-149)

Antitoxin

Vibrio phage ICP1, Orf50

(2-88)


Sequences

Toxin        


Download         Length: 174 a.a.        Molecular weight: 19494.28 Da        Isoelectric Point: 7.9346

>T147430 WP_006809321.1 NZ_CP047729:1685221-1685742 [Enterobacter hormaechei]
VANLTIEMLSEGTDYDFGNFDCGEPSLNAFLTDHLVRQHSGRILRGYLLKERDHPRILGYYTLSGSCFEKAMLPSKTQQR
RIPYSNVPSVTLGRLAVHKELQGNEWGTTLVTHAMRVVYLASQAVGVHGIFVDALNERAKRFYLKLGFIPLAGENSSSLF
FPTQSIERLFEQV

Download         Length: 522 bp

>T147430 NZ_CP062985:53260-53382 [Escherichia coli UTI89]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTGTTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTTGA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10248.70 Da        Isoelectric Point: 5.8836

>AT147430 WP_001303459.1 NZ_CP047729:1684945-1685214 [Enterobacter hormaechei]
MPALKKQRIDLRLSDEDKTMIEEAAAMTNQTITQFMVNSASERAAEVIEQHRRLVLNEASWNAVMDAIDNPPEPNERLKR
AAKRLRNME

Download         Length: 270 bp

>AT147430 NZ_CP062985:53024-53257 [Escherichia coli UTI89]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7T1R089

Antitoxin

Source ID Structure
AlphaFold DB A0A7D7BCZ2

References